摘要
目的探讨细胞自噬状态与子宫内膜异位症之间的关系及其意义。方法对子宫内膜异位症患者在位及异位内膜基质细胞(n=31)及正常子宫内膜基质细胞(n=31)行原代培养,进行免疫细胞化学鉴定;应用透射电镜观察组织中细胞自噬泡超微结构变化;采用RT-PCR、Western blot法检测LC3 mRNA和蛋白的表达。结果透射电镜结果显示:子宫内膜在位及异位内膜细胞的自噬泡数目较少。RT-PCR及Western blot法结果显示:子宫内膜异位症患者在位及异位内膜的LC3 mRNA及蛋白的表达水平明显低于正常子宫内膜(P<0.05)。子宫内膜异位症患者在位与异位内膜的LC3 mRNA表达差异无统计学意义(P>0.05),而异位内膜的LC3蛋白表达水平高于在位内膜(P<0.05)。结论子宫内膜异位症患者的在位及异位内膜基质细胞的自噬活性降低,自噬可能参与子宫内膜异位症的发生。
Purpose To study the relationship between autophagy and endometriosis, and its significance. Methods The eutopic and ectopic endometrial stromal ceils from patients with endometriosis （n = 31 ） and normal endometrial stromal cells （n =31） were cultured primarily and identified by immunocyto-chemistry. The uhrastructural changes of autophagic vacuoles were observed by transmission electron microscopy （TEM）. RTPCR and Western blot methods were used to detect LC3 mRNA and protein expression in endometrial cells of endometriosis patients. Results TEM analysis showed the number of autophagic vacuoles of eutopic and ectopic endometrial cells was fewer. RT- PCR and Western blot analysis showed that the expression of LC3 mRNA and protein in eutopic and ectopic endometrium of patients with endometriosis was significantly lower than that of the normal endometrial stromal cells （P 〈 0. 05）. There was no significant difference in the expression of LC3 mRNA between eutopic and ectopic endometrium of patients with endometriosis （P 〉 0. 05） , but the expression of LC3 protein in ectopic endo-metrium was higher than that in eutopic endometrium of patients with endometriosis （P 〈0. 05）. Conclusion Au/ophagy activity is downregulated in eutopic and ectopic endometrial stromal cells with endometrosis. Autophagy may be involved in the path-ogenesis of endometriosis.
出处
《临床与实验病理学杂志》
CSCD
北大核心
2017年第7期732-736,共5页
Chinese Journal of Clinical and Experimental Pathology
