摘要
该研究以1个疑似柚新品种——‘迷你红柚’为实验材料,利用植物形态学观察、花粉电镜观察以及ISSR分子标记技术,以确定‘迷你红柚’与‘琯溪蜜柚’、‘邓肯葡萄柚’之间的关系。(1)从形态学观察可以看出:‘迷你红柚’果实的外形特征与‘邓肯葡萄柚’较相似,但与‘琯溪蜜柚’有较大的差别;而且‘迷你红柚’嫩叶的形态学特征与‘琯溪蜜柚’和‘邓肯葡萄柚’均有不同。(2)电镜观察发现:‘迷你红柚’花粉的萌发孔沟、花粉沟与‘琯溪蜜柚’和‘邓肯葡萄柚’相似,但花粉大小、外壁网眼与‘琯溪蜜柚’和‘邓肯葡萄柚’均有不同。(3)聚类分析结果表明,‘迷你红柚’和‘邓肯葡萄柚’遗传关系最近,但又有一定距离。研究认为,‘迷你红柚’不同于‘琯溪蜜柚’和‘邓肯葡萄柚’,极有可能是柚的一个新品种。
＇Minihongyou＇, a new pummelo mutant was used as material in this study.Morphological survey, SEM （scan electron microscopy） observation and ISSR molecular marker technology were conducted to confirm the taxonomic status of ＇Minihongyou＇.（1） According to morphological survey results, the ＇Minihongyou＇ fruit is similar with ＇Duncan grapefruit＇, but there were a great of differences between ＇Minihongyou＇ and ＇Guanximiyou＇.Moreover, morphology of young leaves of ＇Minihongyou＇ was different from ＇Guanximiyou＇ and ＇Duncan grapefruit＇.（2） The scanning electron microscopy results showed that pollen size and exine mesh of ＇Minihongyou＇ were distinct with ＇Guanximiyou＇ and ＇Duncan grapefruit＇, howerver, the apertures were the same with the other two species.（3） The cluster results indicated that ＇Minihongyou＇ could be classified into the same group with ＇Duncan grapefruit＇ in a little genetic distance, which means the genetic relationship between ＇Minihongyou＇ and ＇Duncan grapefruit＇ was closer than that with other species.It was concluded that ＇Minihongyou＇ was a novel pummelo, which was different from ＇Guanximiyou＇ and ＇Duncan grapefruit＇.
出处
《西北植物学报》
CAS
CSCD
北大核心
2017年第6期1111-1117,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
福建省科技重大专项(2013NZ0002-1B)
清远市科技计划项目(2015C002)
关键词
柚
孢粉学
形态学
扫描电镜
ISSR分子标记
pummelo
palynology
morphology
scan electron microscopy
ISSR molecular markers
