摘要
目的:观察加味逍遥散对抑郁大鼠行为学及海马组织炎症因子表达的影响,探讨其可能的作用机制。方法:将60只SD大鼠随机分为空白组、模型组、氟西汀组、加味逍遥散低、中、高剂量组,每组10只。除空白组外,其余各组均腹腔注射LPS建立抑郁大鼠模型。氟西汀组每天灌胃盐酸氟西汀1.54 mg/(kg·d),加味逍遥散低、中、高各剂量组每天分别灌胃加味逍遥散2.5 g/(kg·d)、5 g/(kg·d)、10 g/(kg·d),空白组及模型组灌胃等体积生理盐水。通过旷场实验及强迫游泳实验观察大鼠行为学的变化,采用高效液相-质谱联用(HPLC-MS/MS)技术检测海马组织中5-羟色胺(5-HT)含量,实时荧光定量PCR(RT-PCR)检测海马组织IL-1βm RNA、IL-6 m RNA、TNF-αm RNA表达水平。结果:与空白组比较,模型组大鼠的起立数、穿格数、修饰数均明显减少,强迫游泳不动时间延长,海马组织5-HT浓度显著降低,而IL-1βm RNA、IL-6 m RNA、TNF-αm RNA表达显著升高,差异均有统计学意义(P<0.01)。与模型组比较,氟西汀组、加味逍遥散高剂量组大鼠起立数、穿格数、修饰数显著增加,强迫游泳不动时间缩短,海马组织5-HT浓度显著升高,而IL-1βm RNA、IL-6 m RNA、TNF-αm RNA表达量明显降低,差异均有统计学意义(P<0.05,P<0.01)。结论:加味逍遥散对LPS诱导抑郁模型大鼠具有明显抗抑郁作用,通过抑制IL-1βm RNA、IL-6m RNA、TNF-αm RNA的过度表达,增加大鼠海马组织5-HT的含量来改善大鼠抑郁症状。
Objective：To observe the effect of modified Xiaoyao san on ethology and inflammatory factor expression in hippocampus of depression rats,and discuss its potential mechanism. Methods：Selected 60 SD rats and divided them into the blank group,model group,and fluoxetine group,modified Xiaoyao san groups of low dose,medium dose and high dose being 10 cases in each. Except for the blank group,the other groups were all given peritoneal injection with lipopolysaccharide（LPS） to establish depression model rats. The fluoxetine group was given fluoxetine hydrochloride of 1.54 mg/（kg·d） by gavage,the modified Xiaoyao san groups of low dose,medium dose and high dose were given modified Xiaoyao san of 2.5g/（kg·d）,5 g/（kg·d）,10 g/（kg·d） by gavage respectively,and the blank group and the model group were given saline solution of equal volume. Observed change of ethology of rats by open field test and forced swimming test; detected content of5-hydroxytryptamine（5-HT） in hippocampus with high performance liquid chromatography-mass spectrometry, and expression level of Interleukin-1β mRNA（IL-1β mRNA）,Interleukin-6 mRNA（IL-6 mRNA）,tumor necrosis factor-α mRNA（TNF-α mRNA） with real-time PCR（RT-PCR）. Results： Comparing with the blank group, stand up numbers, traversing numbers and modifying numbers of the rats in model group were reduced significantly, and the dead time of forced swimming test was prolonged, 5-HT concentration of hippocampus was reduced significantly,while expression of IL-1βmRNA, IL-6 mRNA, TNF-α mRNA was increased significantly（P〈0.01）. Comparing with the model group, stand up numbers,traversing numbers and modifying numbers of the rats in the fluoxetine group,modified Xiaoyao san groups of low dose, medium dose and high dose were increased significantly, dead time of forced swimming test was shortened,concentration of hippocampus was increased significantly,while expression of IL-1β mRNA, IL-6 mRNA,TNF-α mRNAwas decreased significantly（P〈0.05, P〈
出处
《新中医》
CAS
2017年第7期9-13,共5页
New Chinese Medicine
基金
国家自然科学基金青年基金项目(81503475)
广东省自然科学基金项目(2016A030313491)
广东省中医药局项目(20150153)
