摘要
目的:研究茵陈蒿汤对新生大鼠高胆红素血症的治疗作用及作用机制。方法:200只7日龄SPF级SD大鼠,随机选取36只作为正常组,其余腹腔注射胆红素诱发新生大鼠高胆红素血症,剔除不合格大鼠,选择建模成功大鼠144只随机分为模型组和茵陈蒿汤低、中、高各剂量组,各36只。茵陈蒿汤低、中、高各剂量组分别给予2、4、8 g/(kg·d)茵陈蒿汤灌胃,正常组和模型组灌胃等量的生理盐水,两周为1疗程。观察大鼠的一般行为变化,检测血清和脑组织中胆红素含量和神经元细胞膜Na^+-K^+-ATP酶活力,酶联免疫吸附法(ELISA)检测血清中神经元特异性烯醇化酶(NSE)浓度,TUNEL法检测脑组织细胞凋亡率,逆转录聚合酶链式反应(RT-PCR)检测脑组织caspase-3 mRNA的表达。结果:与正常组比较,模型组和茵陈蒿汤低、中、高各剂量组大鼠给药24 h和14天后血清及脑组织胆红素浓度明显升高,脑组织神经元细胞膜Na^+-K^+-ATP酶的活性明显降低,神经元特异性烯醇化酶含量明显升高,脑组织细胞凋亡率显著升高,差异均有统计学意义(P<0.05,P<0.01)。给药24 h后,与模型组比较,茵陈蒿汤高剂量组大鼠脑组织中胆红素的浓度明显降低,脑组织神经元细胞膜Na^+-K^+-ATP酶的活性明显升高,差异均有统计学意义(P<0.05)。给药14天后,与模型组比较,茵陈蒿汤低、中、高各剂量组大鼠血清及脑组织胆红素浓度明显降低,脑组织神经元细胞膜Na^+-K^+-ATP酶的活性明显升高,神经元特异性烯醇化酶含量和脑组织细胞凋亡率显著降低,差异均有统计学意义(P<0.05,P<0.01)。与同组24 h比较,茵陈蒿汤低、中、高各剂量组给药14 d后,血清及脑组织中胆红素的含量明显降低,脑组织神经元细胞膜Na^+-K^+-ATP酶的活性明显升高,神经元特异性烯醇化酶含量明显降低,差异均有统计学意义(P<0.05,P<0.01)。与正常组比较,模型组大鼠caspase-3 m RNA的表达显著升高(P<
Objective：To study the curative effect and mechanism of Yinchenhao Tang for hyperbilirubinemia on newborn rats. Methods：Two hundreds seven-day-old SPF（Specific Pathogen Free） SD rats were selected as study objects. Among them, 36 rats were divided into the normal group, and the rest were given bilirubin by intraperitoneal injection to induce hyperbilirubinemia. Unqualified rats were eliminated. One hundred and forty-four rats with successful established models were divided into the model group and Yinchenhao tang low,middle and high dosage groups,36 rats in each group. Yinchenhao tang low,middle and high dosage groups were respectively given 2,4 and 8 g/（kg·d） by gavage,while the normal group and the model group were given the same amount of normal saline by gavage. One course lasted for two weeks. Observed general behavioral changes in rats. Detected the bilirubin content in serum and brain tissue as well as the activity ofNa^＋-K^＋-ATPase in neuron membrane. Determined the concentration of serum neuron specific enolase（NSE） by enzyme-linked immunosorbent assay（ELISA） and the ratio of brain cells apoptosis by TUNEL technique. Studied the expression of caspase-3mRNA in brain tissue using reverse transcription-polymerase chain reaction（RT-PCR）. Results：Comparing with those in the normal group,after 24 h and 14 days of medication,in the model group and Yinchenhao tang low,middle and high dosage groups, bilirubin content in serum and brain tissues was evidently increased, the activity of Na^＋-K^＋-ATPase in neuron membrane was markedly reduced,NSE content was significantly raised,and the ratio of brain cells apoptosis was obviously increased,all differences being significant（P〈0.05,P〈0.01）. After 24 h of medication,when compared with those in the model group,in Yinchenhao tang high dosage group,bilirubin content in serum and brain tissue was markedly decreased and the activity of Na^＋-K^＋-ATPase in neuron membrane was raised（P〈0.05）. After 14 days of medication,comp
出处
《新中医》
CAS
2017年第7期1-5,共5页
New Chinese Medicine
基金
河南省科技攻关计划项目(142300410070)
