摘要
目的建立UPLC-MS/MS法同时测定贞芪扶正胶囊(女贞子和黄芪)中腺苷、红景天苷、绿原酸、毛蕊异黄酮葡萄糖苷、特女贞苷、毛蕊异黄酮、芒柄花素、槲皮素、芹菜素、黄芪甲苷的含有量。方法该药物甲醇提取液的分析采用Inertsutain C18色谱柱(75 mm×3.0 mm,2μm);流动相乙腈-甲醇-4 mmol/L乙酸铵,梯度洗脱;体积流量0.5 m L/min;柱温40℃。结果 10种成分在各自范围内线性关系良好(r≥0.996 0),平均加样回收率95.1%~104.3%,RSD小于4.20%。结论该方法快速灵敏,专属性强,可用于贞芪扶正胶囊的质量控制。
AIM To develop a UPLC-MS/MS method for the simultaneous content determination of adenosine, salidroside, chlorogenic acid, calycosin-7-O-β-D-glycoside, speenuezhenide, calycosin, formononetin, quercetin, apigenin and astragaloside IV in Zhenqi Fuzheng Capsules （Ligustri lucidi Fructus and Astragali Radix）. METH- ODS The analysis of methanol extract of this drug was performed on a 40 ℃ thermostatic Inertsutain C18 column （75 mm× 3.0 mm, 2 μm）, with the mobile phase comprising of methanol-acetonitrile-4 mmol/L ammonium ace- tate flowing at 0. 5 mL/min in a gradient elution manner. RESULTS Ten constituents showed good linear rela- tionships within their own ranges （r≥0. 996 0）, whose average recoveries were 95.1% - 104.3% with the RSDs of less than 4.20% . CONCLUSION This sensitive and specific method can be used for the rapid quality control of Zhenqi Fuzheng Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2017年第7期1398-1402,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(21402139)
河北省教育厅优秀青年基金资助项目(YQ2013033)
河北省医学科学研究重点课题计划项目(20150560)
河北省食品药品监督管理局(重点)科技项目(ZD2015023)
