摘要
目的研究程氏蠲痹汤加减方对佐剂性关节炎(AA)大鼠滑膜T细胞受体前列腺素E受体4(PTGER4)水平的影响。方法通过大鼠足跖部皮下注射Freund完全佐剂配合冰水浴和吹风制作AA模型,检测各组大鼠关节肿胀度和关节炎指数,实时荧光定量PCR检测程氏蠲痹汤加减方对滑膜组织PTGER4 mRNA水平的影响。结果程氏蠲痹汤加减方能显著减轻AA模型大鼠关节、滑膜组织的破坏;高剂量(生药含量4 g/mL)处理的大鼠滑膜样本中PTGER4 mRNA水平明显降低。结论程氏蠲痹汤加减方能够降低大鼠AA滑膜组织PTGER4 mRNA水平。
Objective To investigate the effect of modified Cheng＇s Juanbi Decoction on the expression of prostaglandin E receptor 4 （PTGEPA）, the T cell receptor in the synovial tissues, in rats with adjuvant arthritis （AA）. Methods A rat model of AA was established by subcutaneous injection of Freund＇s complete adjuvant at the vola pedis combined with ice-water bath and blowing. The degree of joint swelling and arthritis index were determined in each group. The quantitative real-time PCR was performed to assess the effect of modified Cheng＇s Juanbi Decoction on the mRNA expression of PTGER4 in the synovial tissue. Results Cheng＇s Juanbi Decoction significantly alleviated the damage in the joints and synovial tissues in the AA rats. High-dose （the content of crude drug： 4 g/mL） Cheng＇s Juanbi Decoction significantly reduced the mRNA expression of PTGER4 in the synovial tissues. Conclusion Cheng＇s Juanbi Decoction can reduce the level of PTGER4 mRNA in the synovial tissue in AA rats.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2017年第6期736-740,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81302911)
2013年高校省级优秀青年人才基金(2013SQRL040ZD)
安徽中医药大学自然科学研究项目(2013zr011)
关键词
程氏蠲痹汤加减方
佐剂性关节炎
滑膜组织
T细胞受体表达
modified Cheng＇s Juanbi Decoction
adjuvant arthritis
synovial tissues
T cell receptor expression
