摘要
目的通过建立人牙周膜细胞(hPDLCs)体外张应力加载模型,探讨弱激光照射(LLLI)对受张应力刺激的hPDLCs成骨分化相关蛋白表达的影响。方法取健康青少年(12~16岁)因正畸治疗拔除的双尖牙根中1/3牙周膜组织,进行hPDLCs原代分离培养。取第4代细胞,随机分为空白组(A组)、张应力加载组(B组)和张应力加载并LLLI组(C组),接种于6孔板中。采用免疫印迹法检测加力前及加力后2、6、12、24h时各组细胞成骨分化相关蛋白碱性磷酸酶(ALP)和核心结合因子(Runx2)的表达。结果 C组细胞加力2h时ALP即出现高表达,6h时Runx2出现高表达,而B组细胞两指标则随时间延长表达量逐渐增高,至24h时达到峰值。加力后不同时间ALP、Runx2蛋白表达量C组>B组>A组,各组之间差异有统计学意义(F=7.29~71.19,P<0.05)。结论LLLI能够加快并增强受周期性张应力刺激的hPDLCs成骨分化相关蛋白的表达。
Objective To establish an in vitro tensile stress loading model of human periodontal ligament cells（hPDLCs）,and to investigate the effect of low-level laser irradiation（LLLI）on the expression of proteins involved in osteogenic differentiation of hPDLCs stimulated by tensile stress. Methods One third of periodontal tissue in bicuspid root was collected from healthy adolescents（aged 12-16years）who underwent orthodontic treatment,and primary hPDLCs were isolated and cultured.The fourth-generation cells were divided into three groups：blank group（group A）,tensile stress loading group（group B）,and LLLI＋tensile stress loading group（group C）and inoculated in 6-well plates.Western blotting was used to measure the expression of alkaline phosphatase（ALP）and Runx2 involved in osteogenic differentiation before loading and at 2,6,12,and 24 hours after loading. Results Group C had high expression of ALP at 2hours and high expression of Runx2 at 6hours,while group B had gradual increases in the expression of ALP and Runx2 over the time of loading and had peak expression at 24 hours.At different time points after tensile stress loading,group C had the highest expression of ALP and Runx2,followed by group B and group A（F=7.29-71.19,P〈0.05）. Conclusion LLLI can accelerate and enhance the expression of ALP and Runx2 involved in osteogenic differentiation of hPDLCs stimulated by cyclic tensile stress.
出处
《青岛大学医学院学报》
CAS
2017年第2期168-170,173,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
激光疗法
小剂量
牙周膜
应力
物理
细胞分化
碱性磷酸酶
核心结合因子类
laser therapy
low-level
periodontal ligament
stress
mechanical
cell differentiation
alkaline phosphatase
core binding factors
