摘要
目的对《食品安全性毒理学评价程序》和《保健食品检验与评价技术规范(2003年版)》中的小鼠精子畸形试验进行方法学探讨。方法 30只雄性昆明小鼠完全随机分为3组,空白对照组以蒸馏水灌胃,环磷酰胺组(Cyclophosphamide,CP)分别用国产环磷酰胺和进口环磷酰胺灌胃(40mg/kg.BW),灌胃体积为20ml/kg.BW,每日1次,连续5d,于试验第5天和第35天称重,观察环磷酰胺对小鼠体重的影响。第35天麻醉并处死动物,取双侧睾丸和附睾称重,计算睾丸指数和附睾指数,以附睾研磨涂片,观察精子数量及畸形率情况。结果试验第5天和第35天,CP组小鼠体重均显著轻于空白对照组,差异有统计学意义。但国产CP和进口CP组小鼠体重差异无统计学意义。环磷酰胺组小鼠睾丸重量和附睾重量均显著低于空白对照组,但睾丸指数和附睾指数差异无统计学意义。环磷酰胺显著增加小鼠精子畸形率,精子畸形类型以不定型和无钩为主。结论经口途径给予环磷酰胺使昆明小鼠精子畸形率显著升高,但抑制小鼠体重增长。在进行小鼠精子畸形试验时,应根据体重调整每日灌胃量。阳性药品的选用应综合考虑诱变效果、可获得性和经济性。
Objective To make a methodological study on sperm abnormality test in mice specified in Procedures for Toxicological Assessment of Food and Technical Standards for Testing ＆ Assessment of Health Food （2003 Edition）. Methods Thirty male Kunming mice were randomly divided into three groups. The control group was intragastrieally administrated with distilled water; mice in cyclophosphamide （CP） groups were given home - made and imported CP （40 mg/kg, bw） respectively at a gastric lavage volume of 20 ml/kg, bw, once daily for 5 days. On day 5 and day 35, mice were weighed to investigate the effects of CP on mouse weight. On day 35, animals were anesthetized and killed; bilateral testis and epididymis were isolated and weighed; the testis index and epididymis index were calculated. Epididymis were grinded and smears were prepared to observe the sperm count and abnormalities. Results On day 5 and day 35, the weights of mice in CP groups were significantly lighter than in the control group, and the differences were statistically significant. There was no significant difference in body weight between mice on home - made CP and imported CP. Testis weights and epididymis weights in CP groups were significantly lower than in the control group, but the differences of testis index and epididymis index among the groups were not statistically significant. CP significantly increased sperm abnormality rate in mice; sperm abnormalities mainly included indefinite form and absence of hook. Conclusion CP orally administered significantly increases sperm abnormality rate in Kunming mice but inhibit the body weight gain.In sperm abnormality test in mice, the daily intragastric dose should be adjusted depending on the body weight. The mutagenic effect, availability and cost - effectiveness should be considered in selection of active comparators.
出处
《预防医学情报杂志》
CAS
2017年第8期809-812,共4页
Journal of Preventive Medicine Information
关键词
小鼠
精子畸形
环磷酰胺
方法探讨
mice
sperm abnormality
cyclophosphamide
method exploration
