摘要
目的探讨丙泊酚对心肌梗死大鼠冠状动脉结扎术中心肌的影响。方法 2016年4月,选取50只雄性SD大鼠并随机分为对照组和丙泊酚组,每组25只。两组大鼠均于麻醉满意后开胸,结扎左冠状动脉前降支,制备心肌梗死模型。对照组大鼠给予0.9%氯化钠溶液持续静脉泵注,丙泊酚组大鼠给予丙泊酚持续静脉泵注;建模后1周处死大鼠。记录两组大鼠建模前和结扎冠状动脉后10 min肢体六导联心电图,采用酶联免疫吸附试验检测两组大鼠建模前及建模后1周血清超敏C反应蛋白(hs-CRP)水平,采用TTC染色法检测心肌梗死面积,计算两组大鼠建模后1周心肌梗死面积所占比例;采用TUNEL法检测心肌细胞凋亡情况,计算两组大鼠建模后1周心肌细胞凋亡指数。结果实验过程中对照组5只大鼠死亡,丙泊酚组7只大鼠死亡。两组大鼠建模前心电图正常,两组大鼠结扎冠状动脉后10 min心电图示Ⅰ、Ⅱ、a VL导联ST段抬高、T波高耸或T波与QRS波融合。两组大鼠建模前和建模后1周血清hs-CRP水平比较,差异均无统计学意义(P>0.05);两组大鼠建模后1周血清hs-CRP水平均高于建模前(P<0.05)。建模后1周丙泊酚组大鼠心肌梗死面积所占比例小于对照组(P<0.05)。生物素化d UTP标记阳性的细胞核呈棕褐色,细胞核呈碎点状,不规整,大小不一。建模后1周丙泊酚组大鼠心肌细胞凋亡指数低于对照组(P<0.05)。结论丙泊酚能有效缩小心肌梗死大鼠冠状动脉结扎术中心肌梗死面积、减少心肌细胞凋亡。
Objective To investigate the impact of propofol on myocardium in myocardial infarction rats undergoing coronary artery ligation. Methods In April 2016, a total of 50 male SD rats were randomly divided into control group and test group, each of 25 rats. All of the rats received thoracotomy after successful anesthesia, then left anterior descending coronary artery was ligated to prepare myocardial infarction model. Rats of control group received continuous intravenous pumping of 0. 9% sodium chloride injection, while rats of test group received continuous intravenous pumping of propofol; all of the rats were killed after 1 week of modeling. Six - limb leads electrocardiogram was recorded before modeling and after 10 minutes of coronary artery ligation, enzyme - linked immunosorbent assay was used to detect the serum hs-CRP level before modeling and after 1 week of modeling, TTC staining method was used to detect and calculate the proportion of myocardial infarction area after 1 week of modeling, TUNEL staining method was used to detect and calculate the myocardial apoptosis index after 1 week of modeling. Results During the experiment, 5 rats of control group and 7 rats of test group were dead. Electrocardiogram of the two groups was normal before modeling; after 10 minutes of coronary artery ligation, electrocardiogram of the two groups showed ST - segment elevation in I - , II - and aVL - lead, towering T - wave or integration of T - wave and QRS - wave. No statistically significant differences of serum hs-CRP level was found between the two groups before modeling or after 1 week of modeling （P 〉0. 05） ; after 1 week of modeling, serum hs-CRP level of the two groups was statistically significantly higher than that before modeling, respectively （P 〈 0. 05 ）. Proportion of myocardial infarction area of test group was statistically significantly lower than that of control group after 1 week of modeling （ P 〈 0. 05 ）. Biotinylated dUTP - marked positive cell nucleus were reddish brown,
出处
《实用心脑肺血管病杂志》
2017年第6期38-41,共4页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
关键词
心肌梗死
丙泊酚
冠状动脉
结扎术
大鼠
Myocardial infarction
Propofol
Coronary arteries
Ligation
Rat
