摘要
[摘要] 目的 应用气道纤毛超微结构的综合评价方法,观察香烟烟雾暴露对 COPD 大鼠气道纤毛结构的影响。方法 Wister大鼠,随机分为对照组 （5只）、香烟暴露组 （5只）,香烟暴露组通过 45d被动吸烟建立 COPD大鼠模型,利用光镜观察肺组织病理学变化,电镜观察纤毛超微结构改变, 免疫荧光标记技术观察纤毛超微结 构 中 微 管 蛋 白 变 化。结 果 香 烟 暴 露 组 大 鼠 的 纤 毛 荧 光 强 度 为 （53.46±13.28）,低于对照组 （120.00±26.86） （P ＜0.05）。香烟暴露组纤毛膜水疱数为 （7.36± 2.38）个高于对照组 （0.66±1.21）个 （P ＜0.05）。结论 被动吸烟导致 COPD大鼠气道纤毛结构异常,表现为纤毛膜水疱数增加、纤毛荧光强度减低。
Objective The effects of cigarette smoke exposure on airway ciliary structure in COPD rats were evaluated by the comprehensive evaluation of airway cilia uhrastructure. Methods Wister rats were randomly divided into two experimental groups （CON： control group, n = 5; CS： cigarette smoke group, n =5）. Rats in CS group were exposed to passive smoking for 45 days. The abnormalities of ciliary ultra-structures and the fluorescence intensity of ciliated microtubules were measured by electron microscopy （EM） and confocal microscopy. Results The ciliary uhrastructure abnormalities were found in CS group with typical cilia membrane vesiculation and dynein arm deficiency under EM. The vesiculation in CS group significantly increased （7.36 ± 2.38） compared with CON group （0.66 ± 1.21）. Meanwhile, the microtubule of cilia in CS group decreased, with immune fluorescence intensity was （53.46± 13.28）,compared to CON group （120.00±26.86）（ P 〈0.05）. Conclusions Cigarette smoking could affect the ciliated epithelium in smoking induced airway rats model, with ciliary uhrastruetural abnormalities and absence of cilia microtubules.
作者
冯宇
刘嘉琳
俞婷
孙娴雯
林莹妮
李庆云
FengYu Liu Jialin Yu Ting Sun Xianwen Lin Yingni Li Qingyun(Department of Respiratory Medicine, East Hospital Affiliated to Tongji University, Shanghai 200120, China)
出处
《国际呼吸杂志》
2017年第12期917-920,共4页
International Journal of Respiration
基金
中华医学会临床医学慢性呼吸道疾病科研专项基金（09020610216）
关键词
慢性阻塞性肺疾病
纤毛
微管蛋白
纤毛超微结构
香烟烟雾
Chronic obstructive pulmonary disease
Cilia
Microtubule
Ciliary ultrastructure
Cigarette smoke
