摘要
目的探讨强化剂量阿托伐他汀对不稳定型心绞痛患者经皮冠状动脉介入治疗(PCI)围手术期B7-H3和B7-H4表达的影响。方法将80例不稳定型心绞痛患者随机分为常规剂量组(阿托伐他汀20 mg/d,n=40)和强化剂量组(阿托伐他汀80 mg/d,n=40),分别于PCI术前和术后18~24 h收集外周静脉血,采用ELISA检测外周血白细胞介素4(IL-4)、白细胞介素10(IL-10)、干扰素γ(IFN-γ)、血清可溶性B7-H3(sB7-H3)、可溶性B7-H4(sB7-H4)水平,用实时荧光定量PCR检测外周血单核细胞B7-H3、B7-H4 mRNA相对表达量。结果两组患者PCI术后IL-10、sB7-H3、sB7-H4水平和B7-H3、B7-H4的mRNA表达水平均较术前升高,其中强化剂量组升高更显著(P<0.05)。相反,两组患者PCI术后IL-4、IFN-γ水平均降低,且强化剂量组较常规剂量组下降更明显(P<0.05)。结论强化剂量阿托伐他汀可能通过促进B7-H3、B7-H4表达,从而降低不稳定型心绞痛患者PCI术后免疫炎症反应。
Aim To investigate the effect of intensive dose atorvastatin therapy on B7-H3,B7-H4 in peripheral blood monocytes of patients with unstable angina pectoris undergoing percutaneous coronary intervention （PCI）.Methods The patients with unstable angina pectoris were randomly divided into conventional dose group （atorvastatin 20 mg/d,n=40） and intensive dose group （atorvastatin 80 nag/d,n =40）,peripheral blood were collected before and 18 h ～ 24 h after PCI.Enzymelinked immunosorbent assay was used to detect peripheral blood IL-4,IL-10,IFN-γand sB7-H3,sB7-H4,fluorescence-based quantitive real-time PCR was used to measure B7-H3 and B7-H4 mRNA.Results The levels of IL-10,sB7-H3,sB7-H4 and the expression of B7-H3 and B7-H4 mRNA in the two groups after PCI were higher than those before PCI,and the increase of the intensive dose group was more significant than that of the conventional dose group （P〈0.05）.On the contrary,the levels of IL-4 and IFN-γin the two groups after PCI were decreased,and the decrease of the intensive dose group was more obvious than that of the conventional dose group （P〈0.05）.Conclusion Intensive dose atorvastatin may promote the expression of B7-H3 and B7-H4,thereby reducing the immune inflammation in patients with unstable angina pectoris after PCI.
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第6期590-593,共4页
Chinese Journal of Arteriosclerosis
基金
广西科学研究与技术开发计划项目(科技攻关1355005-4-6)
