摘要
目的使用电喷雾质谱技术,研究3种氨基酸(D-赖氨酸、L-谷氨酰胺和L-酪氨酸)作为手性选择探针对格列美脲和格列美脲异构体的识别效应。方法分别将3种氨基酸与格列美脲及其顺式异构体溶液混合,用电喷雾/离子阱质谱提取非共价结合的复合物,在二级质谱中通过碰撞诱导解离(CID)方法给予不同的碎裂能量,考察碎片离子的丰度从而进行格列美脲及其顺式异构体的识别。结果 L-酪氨酸、D-赖氨酸和L-谷氨酰胺对格列美脲及其顺式异构体均有识别效应,识别率分别为1.61、2.92和2.17。结论以3种手性氨基酸作为立体异构区分探针,首次实现了格列美脲和格列美脲顺式异构体的质谱法快速识别。
Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid （D-Lysine,L-Glutamine and L-Tyrosine） as chiral probes based on electrospray ionization mass spectrometry （ESI-MS） was achieved.Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions.The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation （CID） by MS/MS,which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer.Results Chiral recognition effect was achieved with the recognition rate （R） 1.61,2.92 and 2.17 for D-Lysine,L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds ofchiral amino acids were used as probes to distinguish between stereoisomers,and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.
出处
《药物评价研究》
CAS
2017年第6期792-796,共5页
Drug Evaluation Research
基金
中国食品药品检定研究院青年基金(2014A8)
关键词
顺反异构体
手性氨基酸
电喷雾离子化质谱法
格列美脲
碰撞诱导解离
cis/trans-isomer
chiral amino acid
electrospray ionization mass spectrometry
glimepiride
collision induced dissociation
