摘要
目的:对比观察雌激素、金雀异黄酮、跑台运动、全身垂直振动和氯化锂等5种不同干预疗法对去卵巢骨质疏松大鼠股骨生物力学指标的影响。方法:将80只3月龄雌性SD大鼠按体重分层后随机分为去卵巢(n=68)和假手术(n=12)两个组。手术后第11周,将去卵巢组大鼠按体重分层后又随机分为去卵巢组(11只)、振动组(10只)、跑台组(10只)、金雀异黄酮组(10只)、氯化锂组(10只)和雌激素组(10只),并开始进行不同的干预处理。干预8周时,处死各组大鼠,取右后肢股骨,采用三点弯曲试验检测各组大鼠股骨生物力学指标的变化。结果:(1)雌激素组、金雀异黄酮组、全身振动组、跑台运动组和氯化锂组大鼠股骨结构力学指标中的最大载荷和弹性载荷显著高于去卵巢组(P<0.01,P<0.05),而最大挠度和弹性挠度无显著差异;(2)雌激素组、金雀异黄酮组和全身振动组大鼠材料力学指标中的弹性应力、最大应变和弹性模量显著高于去卵巢组(P<0.05,P<0.01),而最大应力和弹性应变无显著差异。跑台运动组大鼠最大应力、弹性应力、最大应变和弹性模量显著高于去卵巢组(P<0.05,P<0.01),而弹性应变无显著差异。氯化锂组大鼠最大应力、弹性应力和弹性模量显著高于去卵巢组(P<0.05,P<0.01),而最大应变和弹性应变无显著差异。结论:雌激素、金雀异黄酮、跑台运动、全身垂直振动和氯化锂对去卵巢骨质疏松大鼠股骨结构力学指标的干预效果相似,而对材料力学指标的干预效果有一定的差别。
Objective To investigate the effects of different interventions on the biomechanic indexes in femur bones of ovariectomized rats with osteoporosis. Methods Eighty healthy female Sprague-Dawley rats,aged 3 months,were randomly divided into an ovariectomized（OVX,n=68）group and a sham-operation（Sham,n=12）group. At the 11 thweek after the operation,the OVX group was further randomly divided into a control group（n=11）,an estradiol group（E2,n=10）,a genisteine group（G,n=10）,a treadmillexercise group（TE,n=10）,a lithium chloride group（Li Cl,n=10）and a whole-body vertical vibration group（WBVV,n=10）. Then the rats began to receive different interventions as their group names implied.At the end of 8 weeks,their right femurs were isolated and the biomechanic parameters were detectedusing the three point bending test. Results（1）Both the maximum load and elastic load in E2,G,TE,WBVV and Li Cl groups were significantly higher than that of the control group,while no significant differences were seen in maximum deflection and elastic deflection.（2）The elastic stress,maximum strain,and elasticity modulus in E2,G,and WBVV groups were significantly higher than that of the controlgroup,while no significant differences were seen in maximum stress and elastic strain among them. The maximum stress,elastic stress,maximum strain,and elasticity modulus in TE group were significantlyhigher than the control group while no significant differences were seen in the elastic strain. The maximum stress,elastic stress,and elasticity modulus in Li Cl group were significantly higher than the control group,but no significant differences were seen in maximum strain and elastic strain between them.Conclusion E2,genistein,treadmill exercise,whole-body vertical vibration,and Li Cl have quite similar effects on structural mechanics indexes of femurs in OVX rats with osteoporosis,but they have different impacts on the material mechanics indexes.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2017年第6期493-498,共6页
Chinese Journal of Sports Medicine
基金
国家自然科学基金项目(30771046)
北京市百千万人才工程项目(201624)
关键词
去卵巢大鼠
骨质疏松
全身垂直振动
跑台运动
金雀异黄酮
氯化锂
生物力学
ovariectomized rat
osteoporosis
whole-body vertical vibration
treadmill exercise
genistein
lithium chloride
biomechanical
