摘要
通过对蒙古沙冬青[Ammopiptanthus mongolicus(Maxim)Cheng.f]叶组织干旱胁迫下蛋白质组变化的研究,从蛋白表达水平阐释其应答干旱胁迫的分子机制。以20%PEG 6000胁迫处理1 h和72 h,以0 h为对照,提取叶片总蛋白,利用双向电泳和质谱鉴定技术分析差异表达蛋白。共鉴定了40个差异表达蛋白,按功能可分为9类:光合作用,ROS清除,蛋白的合成、加工与降解,物质运输,防御相关,RNA加工,氨基酸代谢,其他相关蛋白和功能未知蛋白质。蒙古沙冬青叶片应答干旱胁迫的核心是叶绿体结构和光合作用的维持。
The aim of the present study is to reveal the proteomic variation of Ammopiptanthus mongolicus（Maxim）Cheng. fleaves under drought stress. The plants were exposed to 20% PEG 6000 for 1 and 72 h,and 0 h as control. The extracted leaves proteinsof the treatment and the control were analyzed by two-dimensional electrophoresis（2-DE）,and the differentially expressed proteins wereidentified by MALDI-TOF-TOF. In total,40 differentially expressed proteins were detected. These identified proteins were mainly involved inphotosynthesis,ROS scavenging,protein synthesis,processing and degradation,transport,defense related,RNA processing,amino acidmetabolism,other and unknown function. Conclusively,the key of A. mongolicus response to drought stress was the maintenance of structureand photosynthesis of chloroplasts.
出处
《生物技术通报》
CAS
CSCD
北大核心
2017年第6期69-80,共12页
Biotechnology Bulletin
基金
国家自然科学基金项目(31370356
31670335)
建设世界一流大学(学科)和特色发展引导专项资金(YDZXXK201619
2015MDTD08C)
中央民族大学研究生科研创新项目(2015)
关键词
蒙古沙冬青
叶片
蛋白质组
差异表达蛋白
Ammopiptanthus mongolicus
leaf
proteome
differentially expressed proteins
