摘要
为探索中药溶液环境对小分子药效成分超滤膜过程的影响及其机理,采用系统模拟的方法,探讨黄连解毒汤中的主要药效成分小檗碱在小分子模拟溶液(模拟溶液A)、小分子+高分子模拟溶液(模拟溶液B)及复方药液中透过率的变化规律及其形成原因.以超滤过程中相对通量变化、小分子物质吸附率、高分子物质的去除率及吸附率为指标,同时测定过程中药液的粒径分布变化、膜阻力分布以及膜表面的电镜扫描(SEM),研究小檗碱在不同溶液环境中的超滤行为,从而探究其透过率差异的形成原因.结果发现,小檗碱在模拟溶液A、模拟溶液B及复方药液中的透过率分别为:(104.68±3.16)%、(86.61±6.13)%、(82.06±6.07)%,吸附率依次为:0%、(1.46±1.09)%、(3.16±1.95)%,对于模拟溶液B和复方药液,蛋白质和果胶的去除率及吸附率无明显差别,淀粉的去除率及吸附率分别为:(68.57±15.65)%、(39.01±3.42)%、(2.8±2.01)%、(17.09±4.22)%,两者均具有显著性差异.由于各药液中物质的颗粒大小及稳定性不同,膜相对通量、粒径分布、阻力分布及SEM均有所差别.对于RC5K超滤膜,影响小檗碱透过率的关键因素是高分子物质,且与其种类、颗粒大小及稳定性相关.
In order to explore the effect and mechanism of solution environment of water decoction of Chinese medical herbs on the ultrafiltration process of micromolecule medicinal ingredients, a system simulation method was used to discuss the variation regularity and forming reasons of transmittance of main effective components namely, berberine from HuanglianJiedu decoction in micromolecule simulation solution （simulation solution A）, micromolecule q- macromolecule simulation solution （simulation solution B） and compound liquid. Using the membrane relative flux, adsorption capacity of micromolecules, together with macromolecules＂ removal efficiency and adsorption rates as guide line. Meanwhile, the particle size distribution, membrane resistance distribution and the scanning electron microscope （SEM） imagines were measured, trying to research the ultrafiltration behavior of berberine in different solution environment in order to find out the causes of transmittance differences of berberine. The results showed that the transmittances of berberine in simulation solution A, B and compound liquid were （104. 68±3. 16）%, （86. 61±6. 13）% and （82. 06±6.07）% respectively, adsorption rates of it were in the order, 0%, （1.46±1.09）% and （3. 16±1.95）% . The removal and adsorption rates of protein and pectin in simulation solution A and compound liquid had no significant difference, but starch in two kinds of liquid had significant differences, their values were （68. 57 ±15. 65）%, （39. 01 ± 3. 42）%, （2. 8 ± 2. 01）%, （17. 09±4. 22）%, respectively. Because of the differences in particle size and stability of the substance in each liquid, the membrane relative flux, particle size distribution, resistance distribution and SEM imagines were all different. For the ultrafihration membrane of RC5K, the key factors affecting the transmittance of berberine is the type, particle size and stability of macromoleeules.
作者
刘静
郭立玮
朱华旭
李博
LIU Jing GUO Liwei ZHU Huaxu LI Bo(Key Laboratory of Separation Engineering for Chinese Medicine Compound,Nanjing University of Chinese Medicine,Nanjing 210029,China Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China)
出处
《膜科学与技术》
CAS
CSCD
北大核心
2017年第3期104-111,共8页
Membrane Science and Technology
基金
国家自然科学基金项目(81274096)
江苏省科技厅重点研发计划-社会发展面上项目(BE2016754)
江苏省六大人才高峰项目(2014-YY-014)
江苏省中药资源产业化过程协同创新中心重点项目(ZDXM-6
ZDXM-8)
关键词
小檗碱
溶液环境
模拟体系
超滤
高分子物质
berberine
solution environment
model system
ultrafiltration
macromolecule materials
