摘要
目的克隆Lactobacillus plantarum LY-78芳香族氨基酸转氨酶基因arat,并对其基因序列进行生物信息学分析。方法利用PCR技术克隆得到芳香族氨基酸转氨酶基因arat的完整序列,测序后运用生物信息学软件预测arat基因对应蛋白的理化性质和结构特征。结果克隆得到了Lactobacillus plantarum LY-78的arat基因,分别命名为arat1和arat2。arat1基因全长为1 188 bp,编码395个氨基酸,蛋白相对分子质量为43 888.9,pI 5.23;arat2基因全长为1 173 bp,编码390个氨基酸,蛋白相对分子质量为43 042.6,pI 5.64;两者均为亲水、热稳定及非分泌型蛋白,具有高度保守性,均属于天冬氨酸转氨酶家族。结论通过基因克隆技术获得了2个arat基因完整序列,为今后Lactobacillus plantarum LY-78 arat基因改造及PLA合成提供了理论支持。
Objective To clone the aromatic amino acid transaminase（arat）gene of Lactobacillus plantarum LY-78 and analyze its bioinformatics. Methods The arat gene was cloned from L. plantarum LY-78 by PCR, then sequenced and predicted the physicochemical properties and structural characteristics of the corresponding protein by using bioinformatic software. Results Both arat genes were successfully cloned from L. plantarum LY-78 and named as arat1 and arat2 respectively. The arat1 gene at a full-length of 1 188 bp encoded 395 amino acids, of which the relative molecular mass and p I were 43 888. 9 and 5. 23 respectively. However, the arat2 gene at a full-length of 1 173 bp encoded 390 amino acids, of which the relative molecular mass and p I were 43 042. 6 and 5. 64 respectively. Both the ARATs are hydrophilic,thermal stable and non-secretory protein, which were highly conserved and belonged to aspartate aminotransferase family.Conclusion Two complete arat gene sequences were obtained by gene cloning, which provide a theoretical support for modification of arat gene of L. plantarum LY-78 and PLA synthesis in future.
作者
李芬
孙大庆
张丽萍
LI Fen SUN Da-qing ZHANG Li-ping(College of Food Science, Heilon~iang Bayi Agricultural University, Daqing 163319, Heilon~iang Province, China)
出处
《中国生物制品学杂志》
CAS
CSCD
2017年第6期587-591,595,共6页
Chinese Journal of Biologicals
基金
黑龙江省青年科学基金项目(QC2014C020)
黑龙江八一农垦大学研究生创新科研项目(YJSCX2016-Y37)
关键词
植物乳杆菌
芳香族氨基酸转氨酶
序列分析
系统进化
Lactobacillus plantarum
Aromatic amino acid transaminase
Sequence analysis
Phyletic evolution
