摘要
[目的]建立并优化‘火焰’品种彩色马蹄莲组培快繁体系。[方法]以‘火焰’品种彩色马蹄莲的球茎嫩芽为试验材料,对其组织培养无性繁殖体系进行研究。[结果]最佳诱导培养基为MS+2.0 mg/L 6-BA+0.2 mg/LNAA;最佳增殖培养基为MS+2.0 mg/L6-BA+0.2 mg/L NAA;植株再生最适合培养基为1/2MS+0.2 mg/L 6-BA+0.2 mg/L NAA;最适生根培养基为1/2MS+0.2 mg/L NAA+2.0 g/L活性炭。[结论]该研究建立了‘火焰’品种彩色马蹄莲的组培快繁体系,为其快速繁殖及产业化生产提供了技术和理论依据。
[ Objective ] To establish and optimze the tissue culture and rapiad progration system of Zantedeschia hybrida ＇ Flame＇. [ Method ] The bulb shoots of Z. hybrida ＇ Flame＇ were used as experimental materials to study the system of tissue culture and rapid propagation. [ Result ] The optimal medium for induction was MS ＋ 2.0 mg/L 6 - BA ＋ 0.2 mg/L NAA. The best proliferation medium was MS ＋ 2.0 mg/L 6 - BA ＋ 0.2 mg/L NAA. The most suitable plant regeneration medium was 1/2MS ＋ 0.2 mg/L 6 - BA ＋ 0.2 mg/L NAA. The optimum rooting medium was 1/2MS ＋ 0.2 mg/L NAA ＋ 2.0 g/L activated carbon. [ Conctustion ] The tissue culture and rapid propagation system of Z. hybrida＇ Flame＇ was established to provide technology and the theoretical fomdation for its rapid propagation and industrialized production.
出处
《安徽农业科学》
CAS
2017年第16期133-135,146,共4页
Journal of Anhui Agricultural Sciences
基金
山东省科技发展计划项目(2013GNC11037)
烟台市科技发展计划项目(2015GNC113)
关键词
'火焰’彩色马蹄莲
组培快繁
优化
Zantedeschia hybrida ＇Flame＇
Tissue culture and rapid propagation
Optimization
