摘要
利用荧光显微镜、扫描和透射电镜对猪细小病毒病(PPV)感染的PK-15细胞进行形态结构观察。结果显示,PPV能诱导猪肾(PK-15)细胞发生典型病变,病变程度随感染时间而加剧。被感染的细胞皱缩、变圆,细胞核浓染、碎裂;超微结构表现为细胞微绒毛消失,有较多的球状突起;粗面内质网严重扩张,线粒体嵴肿胀、模糊不清,核染色质固缩并边集于核膜,在胞核和胞浆内有较多子代病毒聚集;感染的后期出现了细胞膜凹陷包裹胞质成分的凋亡小体,胞浆中细胞器空泡化严重,细胞核碎裂。本研究从细胞水平上了解PPV的致病过程,为其致病机理的研究奠定理论基础。
Abstract：In this study, the ultrastructural changes of PPV--infected PK--15 cells were observed and analyzed by using fluores- cence and electron microscopic technique. Results showed that PPV could cause obviously cytopathic effect, including cell round, chromatin condensation, nuclear fragmentation with a close relation with the PPV infected time. Ultrastructural chan- ges of infected ceils showed cells shrinkaged, reduced in volume with microvilli disappearance and spherical protrusions expan- ding endoplasmic reticulum like a capsule, mitochondria showing hyperplasia, swell, vague, chromatin condensation, or cres- centiform in edge of the nuclear membrane, much progeny virus could be found in the nucleus and cytoplasm At late infected time, formation of membrane embedded apoptotic bodies appeared, cytoplasmic vacuoles increased, organelles severely cavitat- ed, which could not recognized, nuclear fragmentation. This research lays the foundations for the research of pathogenic mech- anism of PPV.
出处
《现代畜牧科技》
2017年第7期1-2,4,共3页
Modern Animal Husbandry Science & Technology
基金
海南省自然科学基金项目(20153086
20153081)
