摘要
目的探讨颗粒蛋白前体(progranulin,PGRN)和卵泡抑素样蛋白1(follostatin-like-protein 1,FSTL1)在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者发病中的作用以及与疾病活动度的相关性。方法采用酶联免疫法吸附试验(ELISA)检测72例SLE患者及36名健康者血清FSTL1及PGRN水平。采用Luminex液相芯片技术检测其中30例SLE患者血清肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素10(interleukin 10,IL-10)水平。采用Mann.Whitney U检验、Spearman等级相关进行统计学分析。结果 (1)血清PGRN、FSTL1在SLE患者表达水平均显著高于健康者(P<0.05);活动组血清PGRN、FSTL1表达水平显著高于稳定组(P<0.05)。(2)SLE患者血清PGRN和FSTL1分别与抗ds-DNA抗体(r=0.275和0.303,P=0.019和0.01)、SLEDAI评分(r=0.341和0.397,P=0.003和0.001)、TNF-α(r=0.411和442,P=0.024和0.014)和IL-10(r=0.452和0.438,P=0.012和0.016)呈正相关,与补体C3呈负相关(r=-0.429和-0.264,P<0.000 1和=0.025)。(3)活动组SLE患者治疗后血清PGRN、FSTL1水平均较治疗前明显降低,差异均有统计学意义(P<0.05);治疗后SLE患者血清中PGRN、FSTL1水平仍高于健康者(P<0.05)。结论 PGRN和FSTL1在SLE的发病机制中扮演着重要角色,可能作为疾病活动的参考指标,有助于指导疾病治疗和预后评估,并为SLE的治疗带来更多靶点。
To investigate the role of progranulin and follostatin-like-protein 1 in the pathogenesis of systemiclupus erythematosus（SLE）, and the correlation between them and disease-activity indexes, serum levels ofprogranulin and follistatin-like-protein 1 in 72 SLE patients and 36 healthy controls were measured by ELISAaccording to the manufacturer＇s instruction; the serum levels of TNF-α and IL-10 were detected by Luminexliquidchip in 30 SLE patients of the 72 patients. And statistical analysis was performed using SPSS 17.0. Thedifferences between two groups were analyzed by using Mann.Whitney U-test; the relations between PGRN orFSTL1 and other variables were analyzed by using the Spearman rank correlation. Data showed that the levels ofserum PGRN and FSTL1 were elevated in patients with SLE compared to the healthy controls（both P〈0.05）; thelevels of serum PGRN and FSTL1 in active SLE patients were higher than those in inactive patients. The levels ofserum PGRN and FSTL1 in SLE patients were both positively correlated with anti-double-stranded DNA antibodytiters（r=0.275 and 0.303, P=0.019 and 0.01）, SLE disease activity index（r=0.341 and 0.397,P=0.003 and 0.001）,TNF-α（r=0.411 and 0.442,P=0.024 and 0.014） and IL-10（r=0.452 and 0.438,P=0.012 and 0.016）, but negatively correlated with serum level of C3（r=-0.429and-0.264, P〈0.000 1 and P=0.025）. After treatment, serum PGRN and FSTL1 were downregulated significantly（P〈0.05）, but still higher than those of the healthy controls（P〈0.05）. The present study demonstrates that PGRN andFSTL1 may play a critical role in the pathogenesis of SLE, of which dynamic monitoring can be used as a referenceindex of disease activity, thus contributing to the treatment and prognosis guide of disease, and lead to moretherapeutics for this disease.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2017年第7期612-618,共7页
Immunological Journal
