摘要
目的分析1型糖尿病初发患儿外周血T细胞亚群(CD4^+、CD8^+、CD4^+/CD8^+)变化特点;探讨1型糖尿病初发患儿外周血T细胞亚群与胰岛功能的关系。方法选择2010-05~2013-12山西省儿童医院确诊的T1DM初发患儿43例,25例健康体检儿童为对照组。采用流式细胞技术测定血清CD4^+、CD8^+、CD4^+/CD8^+T细胞亚群,并对两组结果进行比较。对T1DM初发患儿T细胞亚群与糖化血红蛋白、C肽(C-P)水平进行相关性分析。结果 T1DM组患儿外周血CD4^+T细胞、CD4^+/CD8^+T细胞明显高于对照组(P<0.05),CD8^+T细胞明显低于对照组,差异有统计学意义(P<0.05)。T1DM患儿外周血CD4^+T细胞与Hb A1c呈正相关(r=0.261,P<0.05),与C-P呈负相关(r=-0.270,P<0.05);CD8^+T细胞与Hb A1c无明显相关性(r=-0.156,P>0.05),与C-P水平呈正相关(r=0.162,P<0.05);CD4^+/CD8^+T细胞与Hb A1c无明显相关性(r=0.200,P>0.05),与C-P水平呈负相关(r=-0.243,P<0.05)。结论 T1DM的发病过程存在T淋巴细胞亚群失衡,CD4^+T细胞异常激活、CD8^+T细胞缺陷和CD4^+/CD8^+T细胞比值增高。CD4^+及CD4^+/CD8^+T细胞与C肽呈负相关,CD8^+T细胞与C肽呈正相关。
Objective To investigate the changes of T lymphocyte subsets （ CD4^＋, CD8^＋, CD4^＋/CD8^＋ ） in peripheral blood of un- treated children with type 1 diabetes mellitus, and to explore its relationships with islet function. Methods Forty-three children with T1DM in Shanxi Children＇ s Hospital from May 2010 to December 2013 were selected as experimental group, and 25 healthy children were chosen as control group. The expression of CD4^＋ , CD8^＋ , and CD4 ^＋/CD8^＋ T cell subsets in two groups was measured by flow cytometry, and the results were compared. Correlation between T cell subsets and glycated hemoglobin, C peptide levels in T1DM chil- dren was analyzed. Results The levels of CD4^＋ T cells and CD4^＋/CD8^＋ T cells in peripheral blood were significantly higher in T1DM group than in control group （t = 1. 413, 1. 424, P 〈 0.05）, while the level of CD8^＋ T cells was significantly lower（t = 1. 531, P 〈 0.05 ）. The level of CD4^＋ T cells in peripheral blood was positively correlated with HbA1 c （ r = 0.261, P 〈 O. 05 ）, but negatively correlated with C-P （ r = - 0.270, P 〈 0.05 ）. The level of CD8^＋ T cells was not significantly correlated with HbA1 c （ r = - 0.156, P 〉 0.05 ）, but positively correlated with C-P （ r = 0. 162, P 〈 0.05）. The level of CD4^＋/CD8^＋ T cells was not significantly correlated with HbA1 c （r = 0. 200, P 〉 0.05）, but negatively correlated with C-P（r = - 0. 243, P 〈 0.05 ）. Conclusion T lymphocyte subses imbalance, such as abnormal activation of CIM ＋ T cells, CD8^＋ T cell deficiency and the increase of CD4^＋/CD8^＋ T cell, is involved in the process of the onset of T1DM. CD4^＋ and CD4^＋/CD8^＋ T cells are negatively correlated with C-P, and CD8^＋ T cells are posi- tively correlated with C-P.
作者
张改秀
王蕾
杜建
胡东阳
张明衬
郭婷婷
张晓盼
ZHANG Gaixiu WANG Lei DU Jian HU Dongyang ZHANG Mingchen GUO Tingting ZHANG Xiaopan(Department of Endocrinology, Children＇s Hospital of Shanxi Province, Taiyuan 030013, China Department of Pediatrics, Shanxi Medical Universi- ty)
出处
《山西医科大学学报》
CAS
2017年第6期615-618,共4页
Journal of Shanxi Medical University
关键词
1型糖尿病
T细胞亚群
胰岛功能
糖化血红蛋白
C肽
type 1 diabetes
T cell subset
pancreatic islet function
glycosylated hemoglobin
C peptide
