摘要
为了研究细叶云南松幼苗对不同光照强度的生理响应,以1月生和1年生细叶云南松幼苗为研究对象,采用人工模拟不同光照条件(100%、50.3%、15.0%、4.2%全光照)的方法,对其在不同光照条件下的叶绿素含量、可溶性蛋白含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)等抗氧化酶活性进行研究。结果表明:随着光照强度的减弱,1月生幼苗叶绿素a、b和总叶绿素含量先上升后下降;可溶性蛋白含量在50.3%全光照时最高;SOD、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性以及MDA含量均呈上升趋势,15.0%全光照时达到最大值。随着光照强度的减弱,1年生幼苗叶绿素a、b和总叶绿素含量持续上升;可溶性蛋白含量逐渐下降;SOD活性先上升后下降,15.0%全光照时活性最强;APX、CAT活性以及MDA含量均呈上升趋势,15.0%全光照时达到峰值。1月生和1年生幼苗的POD活性均在50.3%全光照下最大。综合分析得出,50.3%全光照有利于细叶云南松幼苗生理代谢活动的提高,此时,1月生和1年生幼苗的叶绿素和可溶性蛋白含量较高,MDA含量和SOD、CAT等抗氧化酶活性均保持低水平。因此,细叶云南松幼龄时期培育中应适当遮荫。
To study the effects of light intensity on the physiological feature of Pinus yunnanensis var. seedlings, 1 - month-old and 1 - year-old seedlings were planted under different light conditions （ 100% , 50.3% ,15.0% ,4.2% natue sunsine） . A n d their physiological indexes were tested such as the contents of chlorophyll, soluble protein, and antioxidant e n z y m e activities of S O D and so o n. T h e results showed that with the gradual decrease of light intensity, the chlorophyll- a , chlorophyll-b and total chlorophyll c o n-tents of 1 - month-old seedlings increased initially and then decreased. T h e content of soluble protein reached the maximum at 50. 3 % natue sunsine （NS ）. T h e S O D activity, C A T activity, A P X activity and MDA content increased with the decrease of light intensity, and reached the m a x i m u m at 15. 0 % N S . T h e chlorophyll- a , chlorophyll-b and total chlorophyll contents of 1- year-old seedlings gradually increased with the decrease of light intensity, while the soluble protein content decreased gradually. T h e S O D activity in-creased firstly and then decreased, which was the highest under the treatment of 15.0 % N S . T h e C A T ac-tivity, APX activity and MDA content increased with the decrease of light intensity, and the m a x i m u m val-ues were presented at 15. 0 % NS . T h e POD activity of the both seedlings was the highest at 50. 3 % N S . In conclusion,50. 3% NS was beneficial to the increase of physiological metabolic activity of Pinus yurt- nanensis var. seedlings. At this time, the contents of chlorophyll and soluble protein were higher in 1 - month-old and 1- year-old seedlings, and the content of MDA , antioxidant e n z y m e activities of S O D , C A T and so on remained low. Therefore,moderate shade is needed for Pinus yunnanensis var. at juvenility period.
出处
《河南农业科学》
CSCD
北大核心
2017年第6期108-112,共5页
Journal of Henan Agricultural Sciences
基金
广西林业厅科技项目(桂林科字[2016]第20号)
关键词
细叶云南松
光照环境
叶绿素
可溶性蛋白
抗氧化酶
生理响应
Pinus yunnanensis var.
illumination environment
chlorophyll
soluble protein
antioxidant enzyme
physiological response
