摘要
以棕榈硬脂为原料,在脂肪酶Lipozyme TL IM催化作用下,通过分子内重排的形式制备sn-2位富含棕榈酸的甘油三酯,用于后期制备人乳脂替代品的原料。以酯交换产物中sn-2位棕榈酸含量为响应值,考察各反应因素对工艺条件的影响。结果表明:当反应温度为79℃,反应时间为13 h,加酶量为6%(以总底物质量计)时,sn-2位棕榈酸含量可达58.01%,可满足制备人乳脂替代品的原料要求。
Human milk has special triglyceride structure, the main ingredient is 1,3-di-oleic acid-2-palmitic acid triglyceride （OPO）, which means that palmitic acid is mainly distributed on sn-2 position, while the unsaturated fatty acids such as oleic acid, linoleic acid and linolenic acid were mainly distributed on the sn-1,3 position. The present study was to prepare the triglycerides riched in palmitic acid on sn-2 position, which could be used as raw material for the subsequent human milk fat substitute, by enzymatic intra-molecular rearrangement of palm stearin, while lipase Lipozyme TL IM as catalyst. The content of palmitic acid on sn-2 position was used as the response value, and the effects of reaction factors on the process conditions were investigated by single factor experiment and Box-Behnken experimental response surface analysis. The result showed that the optimum reaction conditions were as follows： reaction temperature of 79 ℃, reaction time of 13 h and enzyme load of 6%, under which the content of palmitic acid on the sn-2 position of the obtained glycerides could reach to 58.01%, which could meet the requirements of preparation of human milk fat substitute.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2017年第3期18-24,共7页
Journal of Henan University of Technology：Natural Science Edition
关键词
棕榈硬脂
脂肪酶
分子内重排
sn-2位富含棕榈酸的甘油三酯
palm stearin
lipase
the intra-molecular rearrangement
triglycerides with sn-2 position riched in palmitic acid
