摘要
半日花是半日花科的一种半灌木或灌木,间断分布于我国内蒙古和新疆地区,由于种群数量稀少,现已被列为国家二级保护植物,开展半日花种群遗传多样性和谱系地理结构的研究对于半日花的科学保护具有重要意义。本文基于叶绿体DNA片段trnc-ycf6序列分析和AMOVA发现,半日花种群的遗传变异主要存在于居群间(93%),且居群间遗传分化很高(Gst=0.71,Fst=0.93,Nst=0.92);9个单倍型中4种为内蒙古特有,5种单倍型为新疆特有,有着显著的谱系地理学结构(Nst>Gst)且居群间基因流较低(Nm=0.1),表明半日花种群存在地理隔离,这也是该物种现有单倍型分布格局形成的原因。单倍型最大简约树和网络状树分析,西鄂尔多斯高原可能是半日花冰期"避难所"。
As a subshrub or shrub of Cistaceae, Helianthemum soongoricum has discontinuously distributed in Inner Mongolia and Xinjiang Uigur Autonomous Region in Northwest of China, which has been recommended as the na- tional second- class protective plant due to rare population quantity. It is important to carry out this research for protecting Helianthemum soongoricum scientifically. Based on the trnc - ycf6 sequence analysis of chloroplast DNA fragments and AMOVA analysis, the genetic variation of Helianthemum soongoricum populations mainly existed be- tween the populations （93 % ）, with higher genetic differentiation （ Gst = 0.71, Fst = 0. 93, Nst = 0. 92）. Among 9 haplotypes, 4 haplotypes occurred in NM populations and 5 haplotypes were private for XJ populations, with signifi- cant phylogeographic structure （ Nst 〉 Gst） and low gene flow （ Nm = 0. 1 ）, which indicated there was geographical isolation in Helianthemum soongoricum populations. And this was the reason for forming the distributing pattern of present haplotype. Based on the maximum parsimony tree and network tree analysis, the West Ordos Plateau could be a shelter for Helianthemum soongoricum in ice age.
出处
《内蒙古林业科技》
2017年第2期16-19,共4页
Journal of Inner Mongolia Forestry Science and Technology
基金
国家自然科学基金项目(31060048)资助
关键词
半日花
遗传多样性
谱系地理学
Helianthemum soongoricum
genetic diversity
phylogeography
