摘要
为探讨S100A8和S100A9对鼻咽癌细胞系CNE2的影响及是否通过Wnt/β-catenin通路而发挥作用,以培养基添加1μg/m L S100A8/S100A9培养CNE2为实验组,采用划痕、黏附和平板克隆实验分别检测S100A8/S100A9对CNE2细胞的生物学行为影响,同时运用Western blotting检测CNE2细胞中β-catenin蛋白的累积。实验结果显示,S100A8/S100A9起促进CNE2细胞迁移(p<0.05,p<0.01)、基质黏附(p<0.01)和平板克隆(p<0.01)的作用,且添加S100A8/S100A9蛋白后1 h,CNE2细胞中β-catenin的累积明显上调。以上结果显示S100A8/S100A9可促进鼻咽癌细胞CNE2侵袭和迁移及细胞干性增强等生物学行为,其机制可能有Wnt/β-catenin通路的参与。
To investigate the effect of S 100A8/S 100A9 on CNE2 cells and whether their effects are regulated by Wnt/β-catenin pathway, CNE2 cells cultured with 1 μg/mL S100AS/S100A9 protein were considered as experi- mental group in this study. The effects of S 100A8/S 100A9 on the biological behavior of CNE2 cells were examined through the cell scratch test, cell adhesion assay and plate clone formation assay. Meanwhile, β-catenin protein level in CNE2 cells was analyzed by Western blotting. The results showed that S100A8/S100A9 promoted the ability of migration （,0 〈0.05, p 〈0.01）, adhesion to matrix （p 〈0.01） and increased the number of positive clones （p 〈0.01） of CNE2 cells. Moreover, the accumulation of β-catenin protein in CNE2 cells rose after adding S 100A8/S 100A9 pro- tein for an hour. These results indicated that S 100AS/S 100A9 promoted the invasion, migration, proliferation and the properties of cancer stem cell in CNE2 cells, and the Wnt/β-catenin pathway might involve in the mechanisms.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第6期2215-2220,共6页
Genomics and Applied Biology
基金
国家自然科学基金(No.81260405)
广西自然科学基金(2015GXNSFAA139215)共同资助
