摘要
目的:探讨桦木酸体外拮抗T-2毒素、减少T-2毒素对细胞的氧化应激作用。方法:选用人肝脏细胞L02作为研究对象,将含0.2、2.0、20.0μmol/L的桦木酸和5 nmol/L T-2毒素共同暴露在L02中12h,后测定细胞中SOD、MDA、GSH、GSH-Px、CAT、ALT和AST水平以及细胞培养上清液中的LDH活性,检测细胞氧化损伤情况。结果:随着浓度的提高,桦木酸能剂量依赖地改善由T-2毒素引起的氧化损伤。结论:桦木酸能拮抗T-2毒素,从而改善由T-2毒素引起的氧化损伤。
Objective： To investigate the antagonistic effect of betulinic acid to T-2 toxin in oxidative stress on L02 cells. Methods： The human liver cell L02 was selected as the experimental subject, 0.2, 2.0 and 20 μmol/L of betulinic acid and 5 nmol/L of T-2 toxin was co-exposed to L02 for 12 h, then the level of SOD. MDA. GSH, GSH-Px. CAT, ALT and AST of cells, and LDH activity of culture supernatant were measured to detect cells oxidative damage. Results： It could improve in the oxidative damage in L02 cell induced by T-2 toxin with a dose-dependent relationship. Conclusion： Betulinic acid can antagonize T-2 toxin and improving oxidative damage caused by T-2 toxin.
出处
《中兽医医药杂志》
2017年第3期58-60,共3页
Journal of Traditional Chinese Veterinary Medicine
基金
国家自然科学基金(31302157)
关键词
桦木酸
T-2毒素
氧化应激
betulinic acid
T-2 toxin
oxidative stress
