摘要
目的:探讨姜黄素抑制SW480细胞的作用机制。方法:应用内盐法(MTS)检测方法观察SW480细胞活性及姜黄素的干预影响;应用Western blot、实时定量PCR研究姜黄素对SW480细胞的信号干预机制,检测上皮细胞钙粘蛋白(E-cadherin)、波形蛋白(Vimentin)、β-链蛋白(β-catenin)、T细胞转录因子4(TCF4)基因等表达量。结果:SW480细胞增殖活性在姜黄素干预下被明显抑制,姜黄素对SW480细胞增殖IC50分别为(75.75±3.17)μmol/L(12 h)、(21.15±2.47)μmol/L(24 h)、(18.95±1.75)μmol/L(48 h)、(17.93±1.55)μmol/L(72 h);姜黄素干预下,SW480细胞内E-cadherin、Vimentin明显上调,而β-catenin、TCF4明显下调,且呈剂量依赖性。结论:姜黄素能抑制SW480细胞活性,并通过Wnt/β-catenin信号通路发挥靶向抑制作用。
AIM： To study the effect of curcu- rain on human colon cancer cells SW480. METH- ODS： Cell viability and the effects of curcumin on the activity of SW480 cells were detected by MTS method. Expressions of [3-catenin and TCF4 were detected by Western blot. E-cadherin and Vimentin by real-time qPCR assay in human colon cancer cells SW480. RESULTS： Curcumin could significantly inhibit the proliferation of SW480 cells and the IC50 were （75.75 ±3.17） μmol/L（12 h）, （21.15 ± 2.47）μmol/L （ 24 h）, （ 18.95± 1.75 ） μmol/L（48 h）, （17.93 ±1.55） μmol/L（72 h）. Curcurain could up-regulate the expression of E-eadherin, and down-regulate the expression of Vimentin, β- catenin and TCF4 in SW480 cells. CONCLU- SION： Curcumin can inhibit the the activity of SW480 cells through the Wnt/β-catenin signaling pathway.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2017年第5期526-530,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省中医药资助项目(2015AZ195)
