摘要
本试验以小麦近等基因系TcLr26和Thatcher分别与叶锈菌生理小种260组成不亲和及亲和组合,基于RNA-Seq高通量测序数据库,通过基因表达差异分析,筛选了6个在接种后较0h表达明显变化的属于LRRs类基因的Unigenes,利用实时定量PCR(RT-qPCR)技术对这6个LRRs类基因在接种叶锈菌后的表达进行了分析,为深入研究这些LRRs类蛋白在小麦抗叶锈菌侵染过程中的作用机制奠定了重要试验基础。
The proteins with leucine rich repeats（LRRs）in plant mainly include LRR-RLKs,R proteins and polygalacturonase-inhibiting proteins,which play important roles in plant growth,development,disease resistance and defense response,but only a fewLRRs genes have been identified in wheat.In this experiment,wheat near isogenic lines TcLr26 and Thatcher（hereinafter referred to as Tc）were used to constitute incompatible and compatible combination with Puccinia triticinarace 260 respectively.Based on RNA-Seq database,6Unigenes belonging to the LRRs genes were screened through the analysis of differential gene expression.The expressions of these genes significantly increased at transcription level after inoculation compared to 0hin incompatible combination.In this study,the expressions of 6LRRs genes were analyzed by RT-qPCR after inoculating P.triticina race 260.The result provides the platform for identifying the resistance genes to P.triticinaand the basis for studing wheat resistance mechanism.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2017年第3期32-37,共6页
Journal of Hebei Agricultural University
基金
国家自然科学基金项目(31171472)
高等学校博士学科点专项科研基金资助课题(优先发展领域)(20111302130001)
河北省应用基础研究计划重点基础研究项目(12967149D)
