摘要
该文建立了检测尿液中泛酸含量的液相色谱-串联质谱(HPLC-MS/MS)方法,尿液经过离心、稀释后,采用ACPUITY UPLC SS T3(2.1 mm×100 mm,1.8μm)色谱柱进行分离,电喷雾正离子模式电离,多反应监测模式进行检测,方法的线性关系良好(r=0.999 3),方法检出限为0.46 ng/m L,回收率为87.9%~95.3%,相对标准偏差(RSD)为2.5%~13.0%。该方法具有灵敏度高、分析时间短等特点,可用于尿液中泛酸含量的分析。
A liquid chromatography-tandem mass spectrometric（HPLC-MS/MS）method for the determination of pantothenic acid in urine was developed and validated.After centrifugation and dilution,the sample was separated on an ACPUITY UPLC HSS T3（2.1 mm×100 mm,1.8 μm） column produced by the Waters corporation,and ionized in electro spray positive ion mode,then detected using multi reaction monitoring mode.The calibration curve has a good linear range with a correlation coefficient（r） of 0.999 3.The limit of detection is 0.46 ng/mL,the recoveries are in the range of 87.9%-95.3%,and the RSDs are in the range of 2.5%-13.0%.The method has the advantages of high sensitivity and short analysis time,and could be used to analyze pantothenic acid in human urine.
出处
《分析测试学报》
CAS
CSCD
北大核心
2017年第6期809-811,816,共4页
Journal of Instrumental Analysis
基金
国家自然科学基金项目(21277174)
关键词
液相色谱-串联质谱
尿液
泛酸
生物监测
liquid chromatography tandem mass spectrometry
urine
pantothenic acid
biomonitoring
