摘要
目的研究组蛋白甲基转移酶是否参与了巨噬细胞炎症迁移的过程。方法利用斑马鱼尾巴创伤后巨噬细胞向炎症部位迁移的模型,采用全胚胎原位杂交的方法检测巨噬细胞迁移前后58个斑马鱼组蛋白甲基转移酶的表达变化情况。结果斑马鱼尾巴创伤后,巨噬细胞开始向炎症部位迁移,并在尾巴切除后6 h聚集在尾部的巨噬细胞达到最多。原位杂交结果显示在尾巴创伤后巨噬细胞迁移6 h后,有3个斑马鱼组蛋白甲基转移酶的表达水平有变化。结论斑马鱼组蛋白甲基转移酶可能参与了巨噬细胞炎症迁移的过程。
Objective：The aim of this study was to explore whether histone methyltransferases（HMTs）involve in the inflammatory process of macrophages migration.Methods：We detected the expression levels of the zebrafish 58 histone methyltransferases before or post macrophages migration by whole-mount in situ hybridization（WISH）, using the model of macrophages migration in response to the tail wound.Results：We show that macrophages migrate rapidly to the site of acute injury induced by tail transection, and at 6 hours-post-tail-transection（6 hptt）the number of macrophages that aggregate in the wound reaches a maximum.The expression level of three histone methyltransferases had changed slightly at 6 hours-post-tail-transection.Conclusion：Some histone methyltransferases may play an essential role in macrophages migration in response to inflammation injury in zebrafish.
出处
《泰山医学院学报》
CAS
2017年第2期126-130,共5页
Journal of Taishan Medical College
基金
国家自然科学基金项目(81060212
81160244
81360316
81460283
81550038
81660307
81660204)
内蒙古自然科学基金(2014MS0810
2015BS0801
2015BS0807
2016MS(LH)0307)
包头医学院博士科研启动基金(BSJJ201632
BSJJ201623
BSJJ201617)
内蒙古自治区高等学校科学技术研究项目(NJZY16207)
泰山医学院高层次培育课题(2016GCC02)
