摘要
目的:比较灵芝孢子粉、破壁灵芝孢子粉对Lewis肺癌荷瘤小鼠肿瘤生长及血管内皮生长因子(VEGF)表达的影响。方法:采用C_(57)BL/6小鼠左前肢腋下皮下接种建立Lewis肺癌荷瘤小鼠模型,灵芝孢子粉、破壁灵芝孢子粉各设3、2、1g/kg三个剂量组,连续灌胃给药18天。给药期间观察小鼠体重,记录肿瘤体积变化;末次给药后处死,取肿瘤、胸腺和脾脏,称重并计算抑瘤率、胸腺指数和脾指数;HE染色观察肿瘤组织病理状况;Tunel法检测肿瘤组织细胞凋亡情况;免疫组化法检测肿瘤组织VEGF表达。结果:灵芝孢子粉与破壁灵芝孢子粉均有抑制Lewis肺癌荷瘤小鼠肿瘤的作用,但破壁灵芝孢子粉(3、2g/kg)在提高荷瘤小鼠体重、脾指数、抑制肿瘤生长方面的效果优于灵芝孢子粉;破壁灵芝孢子粉能诱导肿瘤细胞凋亡,降低VEGF蛋白表达。结论:破壁灵芝孢子粉抑制Lewis肺癌荷瘤小鼠肿瘤生长的效果优于灵芝孢子粉,其机制可能与诱导肿瘤细胞凋亡、降低VEGF表达有关。
Objective： To observe the effect of two kinds of Ganoderma lucidum spore powders extract on tumor growth of lung cancer. Methods： Subcutaneous injection of Lewis cell to establish the model of Lewis tumor-bearing mice. Broken ganoderma lucidum spore powders（3,2,1 g/ kg） and ganoderma lucidum spore powders（ 3,2,1 g/kg） were administered for 18 days. During the administration, the weight of the mice and the tumor volume were observed. After 18 days of administration, taken tumors, thymus and spleens from the mice, and calculated the inhibitory rate, thymus index and spleen index. Observed tumor tissue pathological conditions by HE staining. Apoptosis of tumor cells was detected in lung cancer tissues by Tunel method. VEGF protein expression was detected in lung cancer tissues by using immunohistochemical assay method. Results： The effect of broken ganoderma lucidum spore powders （3,2g/kg） is better than ganoderma lucidum spore powders in raising the tumor mice weight, spleen index and inhibiting tumor growth. Broken ganoderma lucidum spore powders can induce tumor cell ap- optosis and decrease the VEGF protein expression. Conlusion： This study reveals the anti-lung cancer effect of broken ganoderma lucidum spore powders may be related to its ability of inducing tumor cell apoptosis and decreasing the VEGF protein expression.
出处
《中药药理与临床》
CSCD
北大核心
2017年第2期118-122,共5页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(81274123)
浙江省重点实验室项目(2012E10002)
关键词
破壁灵芝孢子粉
灵芝孢子粉
肺癌
细胞凋亡
VEGF
Ganoderma lucidum spore powders（灵芝孢子粉）
broken Ganoderma lucidum spore powders（破壁灵芝孢子粉）
lung canc- er
cell apoptosis
VEGF
