摘要
为考察葛仙米(Nostoc sphaeroides)群体粒径变化对营养成分和光合活性的影响,测定了生长过程中不同粒径群体的色素成分、光合特性和多糖含量。结果表明:叶绿素a、藻胆蛋白含量随群体粒径增大而降低;类胡萝卜素含量在粒径4.85 mm时最高,藻蓝蛋白/叶绿素a、藻红蛋白/叶绿素a以及别藻蓝蛋白/叶绿素a的比值随粒径增大先降低后升高;最大潜在光合能力(Fv/Fm)随群体粒径增大逐渐降低,大群体可通过光系统I介导的环式电子传递链耗散多余能量以保护光合作用系统;多糖含量在粒径3.03 mm时最高。研究结果显示葛仙米生长具有明显的尺度效应,细胞组分与光合活性对群体粒径变化具有显著的响应,开展规模化培养和采收需要关注这一效应。
This study investigated nutrients component, photosynthetic activities, pigments components, polysaccha-ride contents and photosynthetic activities during the colony size variation of Nostoc sphaeroides. The result showedthat the content of chlorophyll a and phycobiliprotein decreased with increased colony particle size. The maximum con-tent of carotenoid was occurred in colonies with particle size 4.85 mm; the phycobiliprotein contents was the highest incolonies with particle size 0.77 mm; the maximum content of polysaccharide was occurred in colonies with particle size3.03 mm; the proportion of phycocyanin/chlorophyll a, phycoerythrin/chlorophyll a and allophycocyanin first in-creased and then decreased with the enhanced colony particle size; the maximum potential photosynthetic capacity（Fv/Fm） reduced with the increase of colony particle size. Colonies with smaller size were more tolerant to high light in-tensity than larger ones, and the high rate of cyclic electron transport of the colonies with large particle size can dissi-pate excess energy to counter the damage caused by the high light. The growth of Nostoc sphaeroides shows size effectand the cell component and photosynthetic activities mediate colony size variation, which are important to concern themass of start culture and the harvest time of Nostoc sphaeroides.
作者
赵雪
潘婷婷
米武娟
毕永红
胡征宇
ZHAO Xue PAN Ting-Ting MI Wu-Juan BI Yong-Hong HU Zheng-Yu(The Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China University of Chinese Academy of Sciences, Beijing 100049, China)
出处
《水生生物学报》
CAS
CSCD
北大核心
2017年第4期923-929,共7页
Acta Hydrobiologica Sinica
基金
国家自然科学基金(31123001)
中国科学院璀璨行动计划资助~~
关键词
葛仙米
群体粒径
叶绿素荧光活性
代谢产物
Nostoc sphaeroides
Colony particle size
Photosynthetic activities
Metabolite
