摘要
目的:探讨AMD3100在大鼠短暂性局灶性脑缺血损伤超早期的抗炎作用。方法:将雄性SD大鼠随机分为AMD3100处理组(AMD3100组)、溶剂对照组(PBS组)、假手术组,AMD3100组于再灌注(ischemia-reperfusion,I/R)后给予AMD3100 PBS溶液腹腔注射,假手术组与PBS组于I/R后给予PBS溶液腹腔注射;各组线栓法复制120 min SD大鼠局灶性脑缺血模型,于I/R 24 h后使用ELISA法检测各组缺血区炎症因子IL-10、TNF-α、IL-1β含量。结果:AMD3100组较PBS组IL-10含量高,TNF-α、IL-1β含量低(P<0.05)。结论:AMD3100能够通过抗炎作用对大鼠短暂性局灶性脑缺血损伤起保护作用。
Objective： To explore the anti-inflammatory effect of AMD3100 on the ultra-early time of transient focal brain ischemic injury in rats.Method： Male SD rats were randomly divided into the AMD3100 group, Vehicle group（PBS group） and Sham group, AMD3100 group was given intrapefitoneal injection of AMD3100 after ischemia-reperfusion（I/R）, PDB group and Sham group were given of PBS after I/R.The transiem focal brain ischemia model was induced by Zea-longa modified- suture middle cerebral artery occlusion（MCAO）.Inflammation factors IL-10, IL-1β, TNF-α were detected by using ELISA method.Result： The level of IL-10 was higher and the levels of IL-1β and TNF- a were lower in AMD 3100 group compared with the PBS group（P〈0.05）.Condusion： AMD3100 can improve the prognosis of cerebral ischemia by anti-inflammatory effect.
出处
《中外医学研究》
2017年第17期154-156,共3页
CHINESE AND FOREIGN MEDICAL RESEARCH
