摘要
采用高效液相色谱法(HPLC)对大叶种红茶英红九号的春、夏、秋三季茶的相对保留时间、相对峰面积、共有峰进行了分析.结果表明,HPLC色谱法具有良好的精密度,各样品色谱峰的相对保留时间稳定,可作为指纹图谱的相对时间,确定了7个共有峰.以各色谱峰的峰面积为因子,用相关系数、聚类分析等方法构建了数字化的英红九号红茶HPLC指纹图谱.该方法在区分不同季节生产的英红九号红茶以及分析英红九号红茶与其他品种红茶的差异性等方面提供可能.
High performance liquid chromatography （HPLC） was used to measure black tea samples produced from the large-leaf tea cultivar of Yingde black tea NO.9 in spring, summer and autumn. Their HPLC fingerprints including common peaks, their relative retention times and areas were analyzed. The results showed that HPLC method had good precision, the relative retention times of chromatographic peaks were stable, which could be used to make fingerprints. Seven common peaks were determined in all samples. Using the peak area of each chromatographic peak as a factor, digitized HPLC fingerprint of the Yingde black tea NO.9 was established by the correlation coefficient and cluster analysis method. This method is feasible in discriminating the Yingde black tea NO.9 produced in different seasons and the difference between Yingde black tea NO.9 and other black tea varieties.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2017年第3期37-43,共7页
Journal of South China Normal University(Natural Science Edition)
基金
广东省科技计划项目(2015B090906027,2015A030401065)
英德市科技计划项目(英经信字[2014]65号)
关键词
英红九号
HPLC
指纹图谱
相关系数
聚类分析
Yingde black tea NO.9
HPLC
fingerprint
correlation coefficient
cluster analysis
