摘要
目的:建立了苦参药材中10种黄酮类成分(染料木苷,三叶豆紫檀苷,马卡因,异黄腐醇,苦参醇I,槐黄醇,苦参酮,降苦参酮,2-O-甲基苦参酮及异苦参酮)HPLC测定方法,并对野生与栽培苦参黄酮类成分含量进行比较研究。方法:色谱条件为Kromasil C_(18)色谱柱(4.6 mm×250 mm,5μm),甲醇(A)-0.3%甲酸水(B)为流动相进行梯度洗脱(0~5 min,50%A;5~25 min,50%~70%A;25~35 min,70%A;35~50 min,70%~90%A;50~55 min,90%A),流速0.8 m L·min^(-1),检测波长设定295 nm,数据处理采用独立样本t检验,辅以聚类分析(HCA)和主成分分析(PCA)方法进行评价。结果:独立样本t检验显示,各类成分均表现为P>0.05,即野生品与栽培品在黄酮类含量上无差异;聚类分析和主成分分析也表现为栽培品与野生品苦参黄酮类成分无差异。方法学考察表明10种黄酮类成分具有较好的线性,在其线性范围内r值均>0.999。平均加样回收率在95.0%~105.0%,RSD均在5%以内,符合含量测定的要求。结论:该测定方法快速、准确,可为苦参的质量控制方法提供参考。
Objective： To simultaneously determine the contents of ten flavonoids （genistin, trifolirhizin, maackiain, isoxanthohunol, kushenolI, sophoflavescenol, kurarinone, norkurarinone, 2-O-methyl-kurarinone andisokurarinone） by using HPLC method, compare the flavonoid contents in cultivated and wild Sophorae Flavescentis Radix. Method： The chromatographic separation was performed on Kromasil C18 （4.6 mm × 250 mm, 5 μm） with methanol （A） -0. 3%formic acid solution （B） as the mobile phase for gradient elution （0-5 min, 50% A; 5- 25 min,50%-70% A; 25-35 min, 70% A; 35-50 min, 70%-90% A; 50-55 min, 90% A）. The flow rate was 0. 8 mL.min-I and the detection wavelength was set at 295 nm. Independent t-test （t-test）, hierarchical clustering analysis （HCA） and principal components analysis （PCA） were applied to analyze and evaluate the cultivated and the wild Sophorae Flavescentis Radix. Result： The t-test results showed that P 〉 0.05 for all flavonoids, indicating that there were no significant differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. HCA and PCA also indicated that there were no differences in the contents of flavonoids between cultivated and wild Sophorae Flavescentis Radix. The results showed that ten flavonoids were well separated and had a good linear relationship, with r 〉 0. 999. The average recovery rate was between 95.0%- 105.0%, with RSD 〈 5%, consistent with the content determination requirement. Conclusion： The method is accurate and selective, which could provide reference for the quality control of Sophorae Flavescentis Radix.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第12期58-64,共7页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家“重大新药创制”科技重大专项(2012ZX09103201-009,2012ZX09103201-027)
中药饮片质量保障体系研究(一)项目(201507002)
中国中医科学院客座研究员联合创新研究项目(ZZ070837)
关键词
苦参
黄酮
栽培
野生
Sophorae Flavescentis Radix
flavonoids
cultivated
wild
