摘要
目的:探讨生姜汁和干姜汁炮制黄连对乙醇致大鼠胃黏膜损伤的保护作用差异及其作用机制。方法:将大鼠随机分为7组,即空白组、模型组、生姜汁组、干姜汁组、生黄连组、生姜制黄连组、干姜制黄连组。采用乙醇致大鼠胃黏膜损伤,观察不同组大鼠胃黏膜损伤程度并计算胃黏膜损伤指数,取胃组织制成病理切片,光镜下观察大鼠胃黏膜组织学改变,采用酶联免疫吸附测定法测定血浆中白细胞介素-8(IL-8),肿瘤坏死因子-α(TNF-α)和6-酮-前列腺素F1α(6-keto-PGF_(1α))的含量。结果:与模型组比较,生姜汁组、干姜汁组、生黄连组、生姜制黄连组、干姜制黄连组大鼠胃黏膜损伤指数均显著下降(P<0.01)。与生黄连组比较,生姜制黄连组和干姜制黄连组大鼠胃黏膜损伤指数明显降低(P<0.05),且生姜制黄连组损伤指数较干姜制黄连组更低。血浆中IL-8和TNF-α的含量模型组与空白组相比显著上升(P<0.05),6-keto-PGF_(1α)的含量则显著下降(P<0.05),给药后IL-8和TNF-α含量均下降,6-keto-PGF_(1α)的含量则有所上升,且姜制后作用更强。结论:黄连经过姜汁炮制后可增强其保护胃黏膜损伤的作用,作用机制可能是与抑制IL-8,TNF-α的产生和促进6-keto-PGF_(1α)的产生有关,生姜汁的炮制效果较干姜汁好。
Objective： To explore the differences of inhibition on gastric mucosal injury induced by ethanol in rats between Coptidis Rhizoma （CR） processed with different ginger juice and analyze its mechanism. Method： Rats were randomly divided into the normal group, model group, raw CR group, CR processed by fresh ginger juice （CRFG） group, CR processed by dried ginger juice （CRDG） group, fresh ginger juice （FG） group and dried ginger juice （DG） group. Gastric mucosal injury was induced by alcohol in rats except the normal group. The gastric mucosal injury index was calculated according to the literature scoring method. Histomorphologic changes in gastric mucosa were observed by light microscope. The levels of interluekin-8 （IL-8） and tumor necrosis factor-α （TNF-α） and 6-keto-prostaglandin F1α （6-keto-PGF1α） in plasma were determined by enzyme-linked immunosorbent assay. Result： Compared with the model group, the gastric mucosal injury indexes of FG group, DG group, CR group, CRFG group and CRDG group were decreased significantly. Compared with the CR group, the gastric mucosal injury indexes of CRFG group and CRDG group were decreased significantly, and the injury index of CRFG group was lower than that of CRDG group. The levels of IL-8 and TNF-α in plasma were significantly increased and 6-keto-PGF1α was significantly decreased in the model group by comparing with the blank group. After administration of drugs, the levels of IL-8 and TNF-c~ were decreased and the contents of 6-keto-PGF1α were increased, this effect became stronger after the CR processed with ginger juice. Conclusion： CR processed by ginger juice can enhance the protection of gastric mucosal injury, its mechanism may be through inhibition of production of IL-8 and TNF-α and promotion of production of 6-keto-PGF,~, and FG is better than DG.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第12期18-22,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81260643
81460605)
关键词
黄连
姜黄连
生姜
干姜
胃黏膜损伤
白细胞介素-8
6-酮-前列腺素F1Α
Zingiberis Rhizoma
Coptidis Rhizoma
Coptidis Rhizoma processed by ginger juice
Zingiberis Rhizoma Recens
gastric mucosal injury
interluekin-8
6-keto-prostaglandin F1α
