摘要
目的探讨尼莫地平对大鼠卵巢缺血再灌注损伤后氧化应激水平的影响。方法制作大鼠卵巢缺血再灌注损伤动物模型后,所有大鼠平均分为3组:对照组,模型组和实验组(尼莫地平组1.0 mg/kg),每组12只。HE染色观察大鼠卵巢组织学变化,ELISA法检测大鼠组织内及血清丙二醛(MDA)、一氧化氮(NO)、超氧化物歧化酶(SOD)、髓过氧化物酶(MPO)变化。Western blot检测大鼠卵巢组织中内皮型一氧化氮合酶(e NOS)蛋白表达。结果 ELISA结果显示,模型组大鼠卵巢及血清MDA、NO及MPO含量显著高于对照组(P均<0.05),而实验组大鼠卵巢及血清MDA、NO及MPO含量明显低于模型组(P均<0.05);模型组大鼠卵巢及血清SOD活性显著低于对照组(P均<0.05),而实验组大鼠卵巢及血清SOD活性显著高于模型组(P均<0.05)。Western blot结果显示,模型组中e NOS蛋白表达显著高于对照组(P<0.05),而实验组中e NOS蛋白表达明显低于模型组(P<0.05)。结论尼莫地平能通过抑制氧化应激反应保护大鼠卵巢缺血再灌注损伤。
Objective To investigate the effects of nimodipine （NIM） on the oxidative stress response in ovarian ischemia - reperfusion injury in rats. Methods All rats were divided into 3 groups ： the control group （ n = 12） , the model group （ n = 12） and the test group （ n = 12, NIM 1.0 mg/kg）. Malondialdehyde （MDA） , Nitric oxide （NO） , Superoxide dismutase （SOD） , Myeloperoxidase （MPO） in ovarian and serum were detected by elisa and the protein expression of eNOS in ovarian was valued via western blot. Results ELISA showed that, compared with the control group, the content of MDA, NO and MPO were significantly higher in the model group both in the ovarian and serum （ P 〈 0. 05） , while, the content of MDA, NO and MPO in the test group were markedly lower than that in model group both in the ovarian and serum （ P 〈 0. 0 5） . The activity of SOD in the model group was significantly lower than that in the control group （ P 〈0.05） , while, the activity of SOD in the test group was markedly higher than that in the model group （ P 〈 0.05 ） . Western blot showed that, the protein expression of eNOS in the model group was significantly higher than that in the control group （P〈0.05） , while, the protein expression of eNOS in the test group was markedly lower than that in the model group （ P〈0.05） . Conclusion Nimodipine could protect the ovarian ischemia - reperfusion injury via suppress-ing the oxidative stress response in varian.
出处
《临床和实验医学杂志》
2017年第11期1051-1053,共3页
Journal of Clinical and Experimental Medicine
关键词
大鼠
缺血再灌注损伤
尼莫地平
氧化应激
Rats
Ischemia - reperfusion injury
Nimodipine
Oxidative stress
