摘要
目的:探讨青藤碱(SIN)对小鼠皮肤移植排斥反应的免疫抑制作用。方法:BALB/c小鼠作为供体,C57BL/6小鼠作为受体,建立背-背皮肤移植模型。术后分为假手术组、模型组、SIN组(30 mg·kg^(-1))、环孢素A(Cs A)组(10 mg·kg^(-1))、SIN+亚剂量Cs A组(SIN 30 mg·kg^(-1)+Cs A 5 mg·kg^(-1)),每组10只,腹腔注射给药10d。记录各只小鼠移植皮片的存活天数,术后第4天和第8天采用酶联免疫吸附法(ELISA)检测血浆白细胞介素-2(IL-2)含量。结果:与模型组比较,各给药组动物的移植皮片存活时间均有不同程度的延长(P<0.01);SIN与亚剂量Cs A组的小鼠移植皮片存活时间显著长于SIN组或Cs A组(P<0.05或P<0.01);各给药组第4天和第8天的血浆IL-2水平均较模型组明显降低(P<0.01)。结论:SIN具有抑制皮肤移植小鼠免疫排斥反应的作用,与Cs A联合应用可能具有协同作用。
Objective： To investigate the immunosuppressive effect of sinomenine （SIN） on xenogenic skin transplantation in mice. Methods： BALB/c---~C57BL/6 tergal skin transplantation model was established by an operation, and then the mice were divid- ed into 5 groups at random, namely sham group, model group, SIN group （30 mg· kg-1 ）, ciclosporin A group （CsA） （ 10 mg· kg - l ） and combination of SIN and CsA group （ SIN 30 mg · kg - 1 and CsA 5 mg·kg - l ） （ n = 10 ）. All the mice were intraperitoneally administered once a day for ten days. The survival days of skin graft were recorded, and the IL-2 levels in plasma were determined by ELISA respectively on the 4th and 8th day after the operation. Results ： The mean survival days of skin graft in the groups treated with different drugs were significantly prolonged when compared with that in the model group （P 〈0.01 ）. The combination of SIN and CsA administration showed longer mean survival days than SIN or CsA （P 〈0.05 or P 〈0.01 ）. The IL-2 levels in plasma in the groups treated with different drugs were significantly reduced than those in the model group on the 4th and 8th day after the operation （P 〈 0.01 ）. Conclusion： SIN may have a good immunosuppressive effect in the mice with xenogenic skin transplantation, and the combina- tion of SIN and CsA shows a synergistic effect.
作者
徐丽
张元媛
曹军平
Xu Li Zhang Yuanyuan Cao Junping(Department of Pharmacy, General Hospital of Armed Police Forces, Beijing 100039, China)
出处
《中国药师》
CAS
2017年第6期1028-1030,共3页
China Pharmacist
关键词
青藤碱
免疫抑制
皮肤移植
小鼠
白介素-2
Sinomenine
Immunosuppressive effect
Skin transplantation
Mouse
Interleukin-2
