摘要
目的探讨RhD阴性患者输注DEL型血液的安全性。方法采用间接抗人球蛋白法及热吸收放散方法对RhD阴性献血者血液进行DEL型检测,并采用DNA测序方法分析DEL型献血者的RHD基因序列,回顾性分析接受DEL型血液RhD阴性患者体内抗-D产生情况。结果经吸收放散方法确认的13例DEL阳性的献血者均携带RHD1227A等位基因,12例接受DEL型血液的RhD阴性患者体内抗-D水平均无变化,1例患者体内抗-D水平由8升高到32。结论DEL型血液引起抗-D同种免疫反应的可能性较低,但DEL型血液仍具有一定的免疫原性,对于体内已存在抗-D抗体的患者应避免输注DEL型血液。
Objective To explore the security of DEL red blood cells transfusion to Rh negative recipient. Methods IAT and adsorption-elution were performed on the investigated blood samples to test RhD-negative recipients and DEL phenotype. RHD genotyping were performed with gene sequencing for DEL phenotypes, anti-D alloimmunizatiou was investigated in RhD-negative recipients transfused with DEL red blood cells. Results All the DEL donors were confirmed to carry RHD RHD1227A allele. The titer and strength of anti-D antibodies of 12 true D-negative recipients who were transfused with DEL blood showed no significant difference. However, the titer of anti-D antibodies of one true D-negative recipient increased from 8 to 32.Conclusions Alloanti-D immune response induced by DEL is a rare event in true D-negative recipients. But DEL donors should be excluded for transfusion to D-negative recipients who had carried anti-D.
出处
《临床输血与检验》
CAS
2017年第3期230-233,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
国家自然科学基金(No.30972815)资助
