摘要
目的探讨血性宫颈脱落细胞标本对流式荧光杂交法人乳头瘤病毒(HPV)DNA分型检测结果的影响。方法根据血红蛋白(Hb)水平,将宫颈脱落细胞标本分为5组,A组Hb为0g/L(10例),B组为0g/L<Hb≤30g/L(10例),C组为30g/L<Hb≤60g/L(20例),D组为60g/L<Hb≤100g/L(20例),E组为Hb>100g/L(20例);每组标本中50%初检结果为阳性,50%初检结果为阴性。每例标本分别取样50、100、200、500、1 000μL进行检测,分析检测结果。结果初检结果为阴性的标本、A组5例初检结果阳性标本、B组5例初检结果阳性标本,不同取样量检测结果一致。C、D、E组各10例初检结果阳性标本中,分别有4例、4例和9例标本,在不同取样量检测时,出现HPV亚型漏检。结论血性宫颈脱落细胞标本有可能导致流式荧光杂交法HPV分型检测假阴性结果;有必要根据标本Hb水平,制定合适的取样量标准,以保证检测结果准确性。
Objective To investigate the influence of bloody cervical cell samples on human papillomavirus(HPV)genotyping detection using flowcytometry fluorescence hybridization kit.Methods According to the concentration of hemoglobin(Hb),cervical cell samples were divided into five groups,including group A with Hb of 0g/L(10 cases),group B with 0g/L<Hb≤30g/L(10cases),group C with 30g/L<Hb≤60g/L(20 cases),group D with 60g/L<Hb≤100g/L(20 cases)and group E with Hb>100g/L.In each group,50% samples were with initial positive results of HPV genotyping detection.Every sample was detected using sample volume of 50,100,200,500 and 1 000μL different volumes for testing and analysis results.Results For all samples with initial negative results,five samples with initial positive results in group A and five samples with initial positive results,the detected results of different sample volume were without differences.In the 10 samples,with initial positive results of group C,D and E,there were four,four and nine samples respectively,were with false negative detected results of different sample volumes.Conclusion Bloody cervical cell samples could cause false negative results of HPV genotyping detection using flowcytometry fluorescence hybridization kit.It might be necessary to set optimal sample volume according to the concentration of Hb for ensuring the accuracy of genotyping detection.
出处
《国际检验医学杂志》
CAS
2017年第11期1499-1501,共3页
International Journal of Laboratory Medicine
关键词
人乳头瘤病毒
流式荧光杂交技术
血红蛋白
取样量
human papillomavirus
flowcytometry fluorescence hybridization
hemoglobin
sample volume
