摘要
目的:检测反复下呼吸道感染患者外周血中性粒细胞胞外网状陷阱(NETs)的形成,评价头孢哌酮钠舒巴坦钠对NETs形成的影响。方法:选取反复下呼吸道感染患者36例和健康体检者30名作为病例组和健康对照组。采用激光共聚焦显微镜及扫描电镜检测2组研究对象NETs的形成情况,并根据中性粒细胞的形态将NETs的形成情况分成Ⅰ、Ⅱ和Ⅲ级,计数2组研究对象不同级别NETs形成细胞数量;观察头孢哌酮钠舒巴坦钠处理前后病例组患者NETs的形成情况。结果:病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显多于健康对照组(P<0.05),而Ⅲ级NETs形成细胞数量少于健康对照组(P<0.05);头孢哌酮钠舒巴坦钠处理后病例组患者Ⅰ和Ⅱ级NETs形成细胞数量明显少于用药前(P<0.05),而Ⅲ级NETs形成细胞数量多于用药前(P<0.05)。结论:反复下呼吸道感染患者体内中性粒细胞能形成大量具有高效抗菌功能的NETs,而头孢哌酮钠舒巴坦钠可以抑制NETs的形成。
Objective：To detect the neutrophil extracellular traps （NETs） formation in the peripheral blood of the patients with recurrent respiratory tract infection,and to evaluate the effect of sulbactam sodium/cefoperazone sodium on the formation of NETs.Methods：A total of 36 patients with recurrent respiratory tract infection （case group） and 30 healthy volunteers （healthy control group） were selected.The NETs formation of subjects in two groups was detected by confocal microscope and scanning electron microscope （SEM）.According to the appearance of neutrophils,the formation of NETs was classified as grade Ⅰ,Ⅱ and Ⅲ,the number of NETs formation cells of subjects in two groups was calculated.The formation of NETs of the patients in case group were detected before and after treated with sulbactam sodium/cefoperazone sodium.Results：The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group was more than that in healthy control group （P〈0.05）;while the number of NETs formation cells of grade Ⅲ of the patients in case group was less than that in healthy control group （P〈0.05）.The number of NETs formation cells of grade Ⅰ and Ⅱ of the patients in case group were significantly decreased （P〈0.05）,while the number of NETs formation cells of grade Ⅲ was significantly increased （P〈0.05） after treated with sulbactam sodium/cefoperazone sodium.Conclusion：A lot of NETs with high antibacterial function can be formed in the patients with recurrent lower respiratory tract infection,and sulbactam sodium/cefoperazone sodium can inhibit the formation of NETs.
作者
唐颖
黄元平
荆文花
关英慧
TANG Ying HUANG Yuanping JING Wenhua GUAN Yinghui(Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130021, China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2017年第3期607-610,F0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(20110489)
吉林大学第一医院二部科研专项基金资助课题(B-24)
关键词
反复下呼吸道感染
中性粒细胞胞外网状陷阱
头孢哌酮钠舒巴坦钠
激光共聚焦显微镜
扫描电镜
recurrent lower respiratory tract infection
neutrophil extracellular traps
sulbactam sodium/cefoperazone sodium
confocal microscope
scanning electron microscope
