摘要
目的:观察和分析Goldeneye^(TM) DNA身份鉴定系统的20A试剂盒19个STR基因座在安徽人群亲子鉴定中的突变现象。方法:应用该试剂盒检测1871例亲子鉴定结论为"肯定"的案例并进行基因分型。结果:1871例亲子鉴定中双亲鉴定有66例,单亲鉴定有1805例,共计1937次减数分裂。1871例中共统计到55例有STR基因座发生突变,19个基因座均有突变现象发生。其中一步突变的比例为90.9%。突变来源上,父系和母系来源有明显的性别差异。结论:STR基因座突变是较为常见的现象,要选择突变率低、多态性好的遗传标记,根据实际情况补充检测STR位点以及借助测序等手段以降低误判风险。
Objective：To observe and analyze the mutation phenomenon of 19 STR loci amplified using GoldeneyeTMDNA 20A Kit in paternity test in population in Anhui province.Methods： GoldeneyeTMDNA 20A Kit was used to genotype and verify the genes in 1871 cases with confirmed results in previous paternity test.Results： In 1871 cases,66 received paternity-maternity test and 1805,simple paternity test.A total of 1937 frequencies of meiosis were found,and mutations at STR loci were observed in 55 cases,particularly at the 19 STR loci.One locus mutation accounted for 90.9% in all cases,and there was no difference between the source mutation from paternity and maternity.Conclusion： STR loci mutation is common in population in Anhui province,which suggests that prevention of errors in paternity test should include the alleles with lower mutation at STR loci and genetic markers with better polymorphism as well as additional STR testing and gene sequencing in specific cases.
出处
《皖南医学院学报》
CAS
2017年第3期215-216,220,共3页
Journal of Wannan Medical College
关键词
亲子鉴定
突变
安徽地区
STR基因座
paternity test
gene mutation
Anhui
shot tandem repeat loci
