摘要
目的:检测天然猪链球菌溶血素(n SLY)对人脑微血管内皮细胞凋亡的影响,从而探讨猪链球菌致宿主脑膜炎的发生机制。方法:使用猪链球菌野生株05ZYH33上清、sly基因敲除突变株ΔSLY上清、天然SLY蛋白分别与人脑微血管内皮细胞h CMEC/D3作用4 h后收集细胞,分别用流式细胞术和蛋白免疫印迹技术检测h CMEC/D3细胞凋亡的情况。结果:与对照组比较,天然SLY蛋白可以通过诱导肿瘤坏死因子α(TNF-α)上调显著促进h CMEC/D3细胞发生凋亡,同样含天然SLY的猪链球菌2型野生株05ZYH33上清也通过该途径促进h CMEC/D3细胞凋亡,但突变株ΔSLY上清则不能影响h CMEC/D3细胞的凋亡。结论:猪链球菌溶血素可以通过TNF-α促进人脑微血管内皮细胞发生凋亡,可能是猪链球菌致脑膜炎的发病机制之一。
Objective： To explore the mechanism of meningitis caused by Streptococcus suis, we detect the effect of native S.suis hemolysin（nSLY） on the apoptosis of human brain microvascular endothelial cells.Methods： We used wild strains S.suis 05ZYH33 supernatant, sly knockout mutant strains supernatant and native SLY protein to stimulate the human cerebral microvascular endothelial cells hCMEC/D3 respectively, and collected the cell after 4 h. Furtermore, flow cytometry and protein immunoblot technology were used to observe the apoptosis of the hCMEC/D3.Results： Compared with control group, the natural SLY protein could promote significantly hCMEC apoptosis by inducing the expression of TNF-α. Consisting with the natural SLY protein, the wild strains S.suis 05ZYH33 supernatant also promote hCMEC/D3 apoptosis by the same way, but sly knockout mutant strains supernatant could not affect hCMEC/D3 apoptosis.Conclusion： Suilysin can promote the brain microvascular endothelial cells apoptosis induced by TNF-α, which may be one of the pathogenesis of meningitis caused by S.suis.
出处
《生物技术通讯》
CAS
2017年第3期290-294,共5页
Letters in Biotechnology
基金
国家自然科学基金(81371766)
