摘要
实时定量PCR检测miR-133a-3p在正常胃黏膜细胞GES1和不同恶性程度的胃癌细胞SGC-7901、MGC-803、AGS、HGC-27、BGC-823、MKN-45的表达。miR-133a-3p稳定转染人胃癌细胞系SGC-7901,MTT检测细胞增殖情况,划痕试验观察细胞迁移能力的改变,transwell试验比较细胞侵袭能力的改变。结果表明:正常胃黏膜细胞系中miR-133a-3p的表达显著高于6种胃癌细胞(P<0.05),但其表达高低与分化程度差异不明显(P>0.05)。miR-133a-3p转染SCC-7901细胞后,细胞增殖、迁移和侵袭能力明显降低(P<0.05)。结果说明胃癌的发生发展与miR-133a-3p相关,miR-133a-3p可以作为潜在的胃癌诊断标志。
The expression of microRNA-133a-3p in normal human gastric cell GES1 and six gastric cancer cell lines with different malignant potential were examined by real-time PCR. SGC-7901 was transfected with miR-133a-3p stably. Cell proliferation was measured by MTT assays, the migration was detected by wound assays, and the invasion was com- pared by transwell assays. Results showed that miR-133a-3p expression was shown to be greatly downregulated in human gastric cancer cells compared to normal gastric cells （P〈0.05）, but there was no significant association with the degree of differentiation （P〉0.05）. After transfection with miR-133a-3p, the cell proliferation, migration and invasion were significantly reduced （P〈0.05）. In short, the development of gastric cancer is associated with miR-133a-3p, and miR- 133a-3p can be used as a potential diagnostic marker for gastric cancer.
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2017年第1期30-34,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(81673736)
