摘要
目的研究中药材半夏的丛枝菌根真菌(AMF)的多样性。方法采集浙江杭州(虎跑、下满觉陇、富阳)、贵州贵阳等地半夏及其根际土壤。块茎徒手切片、根剪成1 cm的根段,经10%KOH透明、乳酸甘油酸性品红染液染色、乳酸甘油脱色后,镜下观察半夏植株内AMF的形态结构。湿筛倾析-蔗糖离心法分离半夏根际土中AMF孢子并鉴定,分析各地半夏根际AMF孢子的多样性。结果半夏的皮层组织细胞间及细胞内存在大量菌丝、泡囊,以及典型的丛枝结构。根际土壤中存在大量的AMF孢子。鉴定得到20种3属,其中球囊霉属(Glomus)16种,占80.0%;无梗囊霉属(Acaulospora)3种,占15.0%;盾巨孢囊霉属(Scutellospora)1种,占5.0%。结论各地半夏均可形成丛枝菌根,根际土壤中的AMF孢子具有丰富的多样性,球囊霉属为优势菌群。
Objective To study the diversity of AMF （ Arbuscular mycorrhizal fungi） in pinellia ternate. Method：Pinellia ternate and rhizosphere soil were collected from Hangzhou in Zhenjiang Province （ including Hupao, Xiamanjue-long and Fuyang） and Guiyang in Guizhou Province. The tubers were cut into slices and roots made into lcm long seg-ments, which were colored and decolorized by lactated glycerid acid fuchsin to observe their AMF＇ s morphological struc-ture in plantlets of pinellia ternate. The AMF spores in rhizosphere soils were isolated by the method of wet screeningand sucrose centrifugation and identified to analyze the diversity of the spores from different regions. Results： Therewere large quantities of hypha and vesicle between and inside cells of cortical tissues as well as the typical branchedstructure in pinellia ternate. A lot of AMF spores in rhizosphere soils were being existent. The identification showed thatthere were 20 kinds of pinellia ternate belonging to 3 genera, among which 16 kinds attributes to genus Glomus, 3 to ge-nus Acaulospora and 1 to genus Scutellospora, taking up 80.0%, 15.0% and 5.0% respectively. Discussion： AMFcan be formed in all regions of pinellia ternate and AMF spores in rhizosphere soils are highly diverse, in which the ge-nus Glomus are predominant bacterium.
出处
《陕西中医药大学学报》
2017年第3期75-81,共7页
Journal of Shaanxi University of Chinese Medicine
基金
中医药行业科研专项子课题(201507002-1-11)
关键词
半夏
丛枝菌根真菌
孢子
侵染率
多样性
pinellia ternate, AMF, spores, infection rate, diversity
