摘要
为从蛋白质水平揭示甜高粱适应苏打盐碱胁迫的分子机制,以耐性较强的甜高粱品种M-81E为材料,通过双向电泳和质谱技术鉴定差异表达蛋白质并进行生物信息学分析,同时测定苏打盐碱胁迫下植株抗氧化酶系统活性动态变化。结果表明:在处理条件下,所鉴定的44个差异表达蛋白质有30个上调表达,14个下调表达,主要涉及物质和能量代谢、信号转导及转录调控等功能;GO分析表明,差异蛋白质主要参与氧化还原生物学过程;KEGG富集分析表明,差异蛋白质主要参与碳代谢、光合生物碳固定、糖酵解和次级代谢物生物合成等代谢途径;蛋白质间相互作用直接或间接参与各种代谢通路。苏打盐碱胁迫4 h后,处理组SOD和CAT活性显著下降(P<0.05),GSH-Px酶活性无明显变化,而POD活性极显著升高(P<0.01),并且此时丙二醛和H_2O_2含量较对照组极显著降低(P<0.01),推测在胁迫初期POD是清除活性氧的主效酶;各种抗氧化酶在胁迫16 h时活性呈现不同程度下降趋势。当胁迫24 h时,各种酶活性仍显著高于对照组,但丙二醛和H_2O_2含量剧增。
In order to understand the molecular mechanism of sweet sorghum related to its adap- tation to soda saline-alkali stress at the protein level, the variety M-81E, which has better toler- ance to soda saline-alkali stress, was selected as the material. Various proteins were identified by two-dimensional electrophoresis and mass spectrometry together with the bioinformatics analysis. Dynamic changes of antioxidant enzyme activities in M-81E were detected under soda saline-alka- li stress. Among the identified 44 proteins, 30 and 14 showed up- and down-regulated expres- sions, respectively, which were mainly related to the biological processes of material and energy metabolism, signal transduction and transcription regulation. Gene ontology analysis showed that the differential proteins were mainly involved in redox biological processes. KEGG enrichment analysis showed that the differential proteins were significantly involved in metabolic pathways such as carbon metabolism, carbon fixation in photosynthetic organisms, glycolysis and biosyn- thesis of secondary metabolites. The network of protein-protein interaction showed that proteins in- teracted with each other and directly or indirectly involved in the metabolic pathway. Compared with the control, SOD and CAT activities were significantly decreased （P〈0.05） after soda sa- line-alkali treatment for 4 hours and GSH-Px activity was not significantly changed, while POD activity increased significantly （P〈0.01） in the sweet sorghum seedlings. Correspondingly, contents of MDA and H2O2 in the treated group were lower than those in the control （P〈0.01）. POD could be the key enzyme to scavenge ROS in sweet sorghum at the early stage of soda saline- alkali stress. The activities of various antioxidant enzymes decreased differently after 16 h treatment, and were still higher than the control after 24 h treatment but the contents of MDA and H2O2 increased sharply.
出处
《生态学杂志》
CAS
CSCD
北大核心
2017年第6期1597-1605,共9页
Chinese Journal of Ecology
基金
黑龙江八一农垦大学博士启动基金项目(XDB2013-13)
黑龙江省自然科学基金项目(C2016046)
国家自然科学基金项目(31101194)资助
关键词
甜高粱
苏打盐碱
胁迫
蛋白质组
生物信息学分析
sweet sorghum
soda saline-alkali
stress
proteomic
bioinformatics analysis.
