摘要
目的建立HPLC法同时测定柴仁胶囊(柴胡、酸枣仁、川芎等)中5种成分的含有量。方法该药物80%甲醇提取液的分析采用Merck Purospher STAR C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%甲酸为流动相,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长237、290、335 nm。结果斯皮诺素、阿魏酸、桂皮醛、甘草苷、甘草酸铵分别在0.009 26~0.092 6μg(R^2=0.999 6)、0.056 2~0.562μg(R^2=0.999 8)、0.130~1.30μg(R^2=0.999 8)、0.275~2.75μg(R^2=0.999 5)、0.568~5.68μg(R^2=0.999 2)范围内呈良好的线性关系,平均加样回收率分别为100.73%、100.62%、100.02%、100.41%、99.96%%,RSD分别为2.20%、1.37%、1.52%、1.30%、0.80%。结论该方法简单准确,重复性好,可用于柴仁胶囊的质量控制。
AIM To develop an HPLC method for the simultaneous content determination of five constituents in Chairen Capsules（Bupleuri Radix,Ziziphi spinosae Semen,Chuanxiong Rhizoma,etc.）.METHODS The analysis of 80% methanol extract of this drug was performed on a 30 ℃ thermostatic Merck Purospher STAR C18column（250 mm × 4.6 mm,5 μm）,with the mobile phase comprising of acetonitrile-0.1% formic acid flowing at1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 237,290 and 335 nm.RESULTS Spinosin,ferulic acid,cinnamaldehyde,liquiritin and ammonium glycyrrhizate showed good linear relationships within the ranges of 0.009 26-0.092 6 μg（R-2= 0.999 6）,0.056 2-0.562 μg（R-2= 0.999 8）,0.130-1.30 μg（R-2= 0.999 8）,0.275-2.75 μg（R-2=0.999 5） and 0.568-5.68 μg（R-2=0.999 2）,whose average recoveries were 100.73%,100.62%,100.02%,100.41% and 99.96% % with the RSDs of2.20%,1.37%,1.52%,1.30% and 0.80%,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Chairen Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2017年第6期1191-1194,共4页
Chinese Traditional Patent Medicine
关键词
柴仁胶囊
斯皮诺素
阿魏酸
桂皮醛
甘草苷
甘草酸铵
HPLC
Chairen Capsules
spinosin
ferulic acid
cinnamaldehyde
liquiritin
ammonium glycyrrhizate
HPLC
