摘要
目的:探讨促性腺激素释放激素(GnRH)对人鼻咽癌CNE2细胞活力、凋亡、迁移和侵袭能力的影响。方法:收集鼻咽癌和慢性鼻咽炎症的组织标本,采用免疫组织化学染色、qRT-PCR分析检测标本中GnRH阳性细胞、GnRH mRNA的表达。体外培养人鼻咽癌CNE2细胞,永生化鼻咽上皮细胞株NP69,采用免疫组织化学染色,qRT-PCR分析检测细胞中GnRH阳性细胞、GnRH mRNA的表达。以不同浓度0(空白对照组)、10^(-2)、10^(-1)、10~0nmol/L GnRH处理CNE2细胞,采用CCK-8、流式细胞术、划痕修复实验、Transwell小室体外侵袭实验观察GnRH对CNE2细胞活力、凋亡、迁移和侵袭能力的影响。结果:鼻咽癌组织中GnRH阳性细胞、GnRH mRNA表达明显低于慢性鼻咽炎症组织(P<0.05)。CNE2细胞中GnRH阳性细胞、GnRH mRNA表达明显低于NP69(P<0.05)。与空白对照组比较,GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力(P<0.05或P<0.01)。结论:GnRH显著抑制CNE2细胞活力、凋亡、迁移和侵袭能力。
Objective：The aim of this study is to investigate the effect of gonadotropin releasing hormone （Gn- RH） on suppressing cell viability, apoptosis, migrationg and invationg of human nasopharyngeal carcinoma cells CNE2. Method：Nasopharyngeal carcinoma tissues and postnasal catarrh tissues were collected, the expression of GnRH positive cells and GnRH mRNA were detected by immunohistochemical staining and qRT-PCR. The human nasopharyngeal carcinoma CNE2 cells and immortalized nasopharyngeal epithelial cell line NP69 were cultured in vitro,and the expression of GnRH positive cells and GnRH mRNA were detected by immunohistochemical staining and qRT-PCR. The CNE2 ceils were treated with GnRH with various concentrations 0 （Blank group）, 10^-2, 10^-1 , 10^0 nmol/L. The effects of GnRH on the viability, apoptosis, migration and invasion of CNE2 cells were de- tected by cell Counting Kit（CCK-8）, flow cytometry, wound healing assay and transwell chamber assay in vitro. Result：The expression of GnRH positive cells and GnRH mRNA in nasopharyngeal carcinoma tissues were markedly down regulated than postnasal catarrh tissues（P〈0. 05）. The expression of GnRH positive cells and GnRH mRNA in CNE2 cells were markedly down regulated than NP69 cells （P〈 0.05）. Compared with blank group, GnRH can significantly inhibite the cell viability cells, apoptosis, migration and invasive ability（P〈0.05 or P〈 0.01）. Conclusion：GnRH significantly inhibited the cell viability, apoptosis, migration and invasive ability of CNE2 cells.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2017年第11期830-834,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
促性腺激素释放激素
鼻咽肿瘤
迁移
侵袭
gonadotropin-releasing hormone
nasopharyngeal neoplasms
migration
invasion
