摘要
目的探讨内皮素拮抗剂对紫外线诱导豚鼠色素沉着的治疗作用。方法用中波紫外线（UVB）照射豚鼠皮肤制作色素沉着模型。模型制作成功后，模型动物分为空白对照组（生理氯化钠溶液）、内皮素拮抗剂组及熊果苷组（阳性对照）。分别于中波紫外线（UVB）照射前、15d后及30d后，用MexameterMX18检测豚鼠背部皮肤的黑素指数。连续治疗30d后比较黑素指数、表皮内黑素细胞数目和黑素含量等指标。结果UVB照射30d时，豚鼠背部9块UVB照射区域的黑素指数显著高于照射前（P〈0．0001）。外涂1‰内皮素拮抗剂的照射区域30d后的黑素指数值明显降低，和熊果苷组比较，差异有统计学意义（P〈0．0001）。内皮素拮抗组黑素含量指数1明显低于空白对照组（P〈0．05）。而3组间黑素含量指数2差异无统计学意义（P〉0．05）。结论外涂内皮素拮抗剂对UVB照射诱导豚鼠的色素沉着有一定治疗作用。
Objective To evaluate the therapeutic effect of endothelin antagonist on ultraviolet B （UVB）-induced pigmentation in guinea pigs. Methods A skin pigmentation model was established by UVB irradiation in guinea pigs. The modeled animals were divided into 3 groups to be treated with sodium chloride physiological solution （blank control group）, an endothelin antagonist （endothelin antagonist group） and arbutin （positive control group）, respectively. Before the UVB irradiation, as well as after 15- and 30-day UVB irradiation, Mexameter MX lg was used to detect the melanin index in the dorsal skin of guinea pigs. After 30-day consecutive treatment, melanin index, the number of melanocytes in the epidermis and melanin content were compared among the above 3 groups. Results After 30-day UVB irradiation, the melanin index in the 9 UVB- irradiated areas on the dorsal skin of guinea pigs significantly increased compared with that before the radiation （P 〈 0.0001）. After 30-day treatment with the 1‰ endothelin antagonist, the melanin index in the UVB-irradiated areas significantly decreased compared with that in the positive control group （P 〈 0.0001）. Melanin content index-1 （MCI-1） was significantly lower in the endothelin antagonist group than in the blank control group （P 〈 0.05）, while no significant difference in MCI-2 was observed among the 3 groups （P 〉 0.05）. Conclusion Topical application of the endothelin antagonist has a certain therapeutic effect on UVB-induced pigmentation in guinea pigs.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2017年第6期436-439,共4页
Chinese Journal of Dermatology
基金
泉州市财政局科技计划项目（2013270）
关键词
紫外线
色素沉着
治疗应用
豚鼠
内皮素拮抗剂
Ultraviolet rays
Pigmentation
Therapeutic uses
Guinea pigs
Endothelin antagonist
