摘要
【目的】荧光原位杂交技术可以实现DNA序列直观准确的染色体定位,是基因组深入研究的重要技术之一。染色体特异探针的获得是该技术应用的关键。本研究旨在建立棉花寡核苷酸荧光原位杂交技术。【方法】利用已经公布棉花基因组序列数据,采用生物信息学方法获得染色体特异的寡核苷酸库,随后用乳化聚合酶链式反应方法标记成荧光探针,在棉花有丝分裂中期染色体上进行原位杂交。【结果】建立了一套棉花寡核苷酸荧光原位杂交技术体系。【结论】该体系可用于棉花单染色体识别鉴定。
[Objective] Fluorescence in situ hybridization （FISH） can achieve the accurate chromosomal localization of DNA sequences, which is one of the important techniques for genome in-depth study. The acquisition of chromosome-specific probes is the key for FISH application. The aim of this study is to explore a system of cotton oligonucleotides （oligos） fluorescence in situ hybridization （Oligo-FISH） technology. [Method] Based on the published cotton genome sequence, the chromosome-specific oligos library was obtained by bioinformatics method. Then, the obtained oligos were labeled through emulsion polymerase chain reaction, and used for FISH of cotton mitotic chromosome. [Results] A system of cotton Oligo-FISH technology was set up and successfully applied in cotton chromosome identification. [Conclusion] This system can be used for dissection of cotton genomes.
出处
《棉花学报》
CSCD
北大核心
2017年第3期213-221,共9页
Cotton Science
基金
国家自然科学基金(31471548)
棉花生物学国家重点实验室开放课题(CB2017A06)
安阳工学院博士科研启动基金(BSJ2016005)
关键词
棉花
寡核苷酸
荧光原位杂交
染色体
cotton
oligos
fluorescence in situ hybridization
chromosome
