摘要
目的:探讨电针百会、神庭穴对脑缺血再灌注损伤大鼠学习记忆能力及突触可塑性的影响。方法:将36只清洁级雄性SD大鼠随机分为假手术组、模型组和电针组,其中12只仅分离血管作为假手术组,其余24只运用线栓法制备左侧大脑中动脉局灶性脑缺血再灌注大鼠模型,缺血时间为2 h。造模成功后采用随机数字表法分为模型组和电针组各12只。电针组大鼠给予电针百会、神庭穴,1次/d,持续7 d;其余2组同等条件抓取,不予干预。各组大鼠在造模成功后第3天开始采用Morris水迷宫检测大鼠学习记忆能力,水迷宫试验共持续5 d;采用透射电镜观察海马区突触超微结构的变化;免疫组化法检测突触后致密蛋白95(PSD-95)和突触囊泡膜蛋白-突触素(SYN)的表达。结果:水迷宫测试中,模型组与假手术组相比逃避潜伏期明显增加(P<0.001),穿越平台次数减少(P<0.01);电针组与模型组比较逃避潜伏期缩短(P<0.01),穿越平台次数增加(P<0.05),第5天的穿越平台轨迹较集中于平台附近,而模型组则较分散和不规则。通过透射电镜观察,模型组与假手术组相比突触数量显著减少(P<0.001);电针组比模型组海马CA1区突触的结构更加清晰和完整,数量显著增加(P<0.01)。免疫组化发现假手术组比模型组海马区PSD-95及SYN表达显著增高(P<0.001);与模型组相比,电针组海马区的PSD-95表达明显增高(P<0.001),SYN表达也显著增高(P<0.01)。结论:电针百会、神庭穴可以改善脑缺血再灌注损伤大鼠学习记忆能力,其机制可能与增强突触可塑性,包括促进突触结构的完整,增加PSD-95和SYN的表达有关。
Objective：To observe the efficacy of electroacupuncture at Baihui （DU20） and Shenting （DU24） acupoints on learning and memory and synaptic plasticity in rats after cerebral ischemia/reperfusion （I/R） injury. Methods：Thirty-six male Sprague-Dawley rats were randomly divided into the sham group, the ischemia/reperfusion （I/R） group and the electroacupuncture group after surgery. Twenty-four rats were performed the focal cerebral ischemia and reperfusion injury on the left middle cerebral artery by suture method for 2 hours and another 12 rats were subjected to separation of vessels. The 24 rats were modeled with middle cerebral artery occlusion （MCAO） for 2 hours and then reperfusion. The rats of electroacupuncture group received electroacupuncture at Shenting and Baihui acupoints once a day for 7 days. Rats in another 2 groups were only grabbed under the same condition without intervention. Learning and memory ability were tested by Morris water maze. The maze was started at 3th and lasted for 5 days. The changes of synaptic ultrastructure in the hippocampus were observed by transmission electron microscope. Immunohistochemistry was used to test the expression of postsynaptic density protein 95 （PSD-95） and synaptophysin （SYN） in the hippocampus. Results：Com- pared with the I/R group,the latency of water maze decreased and the times crossing the platform increased in the sham group （P〈0.001） and the electroacupuncture group （P〈0.01）. Trajectory crossing platform on the 5th day focused on the platform while the trajectory-crossing platform in the I/R group was irregular. The structure of the hippocampus CA1 area of the electroacupuncture group was more clear and complete. And compared with the I/R group,the sham group and the electroacupuncture group increased the expression of PSD-95 in hippocampus （P〈 0.001 ）. Compared with the I/R group, the expression of SYN in hippocampus in- creased in the sham group （P〈 0.001 ） and the electro acupuncture group （P�
出处
《康复学报》
2017年第1期29-35,共7页
Rehabilitation Medicine
基金
国家自然科学基金项目(81403462)
福建省教育厅资助省属高校项目(JK2014022)
